Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Arch Oral Biol. 2023 Jun;150:105687. doi: 10.1016/j.archoralbio.2023.105687. Epub 2023 Mar 17.
To evaluate the composition and function of mural cell populations in human gingival tissues DESIGN: A cross-sectional study was conducted on seven periodontitis (stage Ⅲ) patients. Gingival tissues were collected two months after scaling and root planing and divided into 3 groups: 1, h_h group (horizontal bone resorption, residual pocket depth ≤3 mm); 2, v_h group (vertical bone resorption >4 mm, residual pocket depth ≤3 mm); 3, v_i group (vertical bone resorption >4 mm, residual pocket depth ≥6 mm). Single-cell RNA sequencing (10X genomics) and subsequent bioinformatics analysis were performed. Protein expression of selected genes was confirmed by histological staining.
Two mural cell clusters, RGS5THY1 and ACTA2MYH11 subpopulations, were identified and confirmed by histological staining and cross-validation with three different single-cell RNA sequencing datasets in the GEO database. RGS5THY1 cluster in perivascular areas possessed cellular protrusions and exhibited immunomodulatory and synthetic phenotypes. In contrast, the ACTA2MYH11 cluster strictly distributed around vessel walls was characterized by a contractile phenotype. Mural cells closely interacted with endothelial cells through PDGF and NOTCH3 signaling. Mural cell loss was detected in the v_i group and in hopeless periodontal teeth, which might be caused by tumor necrosis factor-alpha induced apoptosis.
Gingival mural cells can be classified into two distinct clusters according to their gene signatures and cell morphology. The loss of mural cells may indicate periodontitis progression.
评估人牙龈组织中壁细胞群体的组成和功能。
对 7 名牙周炎（Ⅲ期）患者进行横断面研究。在牙周刮治和根面平整后两个月收集牙龈组织，并将其分为 3 组：1、h_h 组（水平骨吸收，残留袋深度≤3mm）；2、v_h 组（垂直骨吸收>4mm，残留袋深度≤3mm）；3、v_i 组（垂直骨吸收>4mm，残留袋深度≥6mm）。进行单细胞 RNA 测序（10X 基因组学）和随后的生物信息学分析。通过组织学染色证实所选基因的蛋白表达。
通过组织学染色和与 GEO 数据库中三个不同的单细胞 RNA 测序数据集的交叉验证，鉴定并确认了两个壁细胞簇，RGS5THY1 和 ACTA2MYH11 亚群。RGS5THY1 簇位于血管周围区域，具有细胞突起，并表现出免疫调节和合成表型。相比之下，严格分布在血管壁周围的 ACTA2MYH11 簇表现出收缩表型。壁细胞通过 PDGF 和 NOTCH3 信号与内皮细胞密切相互作用。在 v_i 组和无望牙周牙中检测到壁细胞丢失，这可能是由肿瘤坏死因子-α诱导的细胞凋亡引起的。
根据基因特征和细胞形态，牙龈壁细胞可分为两个不同的簇。壁细胞的丢失可能表明牙周炎的进展。
