Department of Veterinary Gynaecology and Obstetrics, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura 281001, Uttar Pradesh, India.
Sperm Signalling Laboratory, Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura 281001, Uttar Pradesh, India.
Anim Reprod Sci. 2023 Apr;251:107214. doi: 10.1016/j.anireprosci.2023.107214. Epub 2023 Mar 15.
In the study, melatonin, a known antioxidant pineal peptide was used as an additive in the tris-egg yolk glycerol-based semen extender in Hariana bull semen and post-thaw sperm characters were evaluated. In the study, Group I was a control without melatonin; and Group II, III, and IV were having 0.5 mM, 1 mM, and 2 mM melatonin/80 × 10 spermatozoa, respectively were treatment groups. Thirty-two semen ejaculates from 4 Hariana bulls were processed for freezing and post-thaw sperm characteristics were evaluated. Sperm motility, velocity, the viability with intact membrane, and total antioxidant capacity were markedly (P < 0.05) improved in Group IV compared to all other groups. The lipid peroxidation and total protein carbonylation were substantially (P < 0.05) decreased in Group IV compared to all other groups. The population of cryocapacitated, acrosome-reacted, and apoptotic-like spermatozoa were significantly (P < 0.05) decreased in Group IV. Further, the relative band intensity of 74 kDa protein and percent of spermatozoa showing positive immune reactivity to tyrosine-phosphorylated proteins was decreased in Group IV. The progesterone-receptor ligand binding, in vitro capacitation response, and Vanguard distance were markedly (P < 0.05) improved in Group IV. In summary- Group IV having 2 mM melatonin was found to be optimal in providing cryoprotective effects to Hariana bull spermatozoa after freezing-thawing and can be suitably used as a semen additive during semen cryopreservation.
在这项研究中，褪黑素，一种已知的抗氧化松果腺肽，被用作哈里亚纳公牛精液中 tris-卵黄甘油基精液稀释液的添加剂，并评估了解冻后精子的特性。在这项研究中，第 I 组为不含褪黑素的对照组；第 II、III 和 IV 组分别为添加 0.5mM、1mM 和 2mM 褪黑素/80×10 个精子的处理组。对 4 头哈里亚纳公牛的 32 份精液进行冷冻处理，并评估解冻后精子的特性。与其他所有组相比，第 IV 组的精子活力、速度、膜完整性的活力和总抗氧化能力明显（P<0.05）提高。与其他所有组相比，第 IV 组的脂质过氧化和总蛋白羰基化显著（P<0.05）降低。冷冻能力、顶体反应和凋亡样精子的数量在第 IV 组显著（P<0.05）减少。此外，第 IV 组的 74kDa 蛋白相对带强度和显示酪氨酸磷酸化蛋白免疫反应阳性的精子百分比降低。孕激素受体配体结合、体外获能反应和 Vanguard 距离在第 IV 组明显（P<0.05）改善。总之，第 IV 组添加 2mM 褪黑素对哈里亚纳公牛精子冷冻解冻后具有最佳的保护作用，可作为精液冷冻保存期间的合适添加剂。
