Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa VigyanViswavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India.
Sperm Signalling Laboratory, Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa VigyanViswavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India.
Andrologia. 2022 Feb;54(1):e14255. doi: 10.1111/and.14255. Epub 2021 Sep 27.
In this study, the cryoprotective potential of natural antioxidant curcumin in Hariana bull semen was evaluated as an additive in a tris-based extender with the assessment of motility and motion parameters of spermatozoa, membrane intactness, progesterone-receptor binding, protein carbonyl content, cervical mucus penetration, cryocapacitation-associated and apoptotic-like changes. The collected ejaculates were divided into five groups in the tris-based extender (control without curcumin-I, 10 µM-II, 25 µM-III, 50 µM-IV and 75µM-V) and were cryopreserved. Groups II and III containing 10 and 25 µM curcumin substantially (p < .05) improved the post-thaw sperm parameters like viability, motility, and velocity parameters; intact acrosome and membrane; lowered protein carbonyl content; DNA fragmentation and cryocapacitation-associated changes in comparison to control. It was interesting to note that early apoptotic-like changes in sperm cells were significantly (p < .05) decreased in Group II along with an increase in a higher population of sperm cells having high mitochondrial transmembrane potential. Higher progesterone-receptor binding, Vanguard distance and in vitro capacitation response were observed only in Group II (10µM) compared to other groups. In conclusion, curcumin in a semen extender manifests cryoprotective effects and may be incorporated at 10 µM concentration in a Hariana bull semen extender for better post-thaw sperm quality.
在这项研究中，评估了天然抗氧化剂姜黄素作为添加剂在 tris 基础 extender 中的抗冷冻保护潜力，同时评估了精子的运动和运动参数、膜完整性、孕酮受体结合、蛋白质羰基含量、宫颈粘液穿透、冷冻诱导的顶体反应和凋亡样变化。收集的精液在 tris 基础 extender 中（不含姜黄素的对照组-I、10µM-II、25µM-III、50µM-IV 和 75µM-V）分为五组并进行冷冻保存。含有 10 和 25µM 姜黄素的 II 组和 III 组显著（p<.05）提高了解冻后精子的参数，如活力、运动和速度参数；完整的顶体和膜；降低了蛋白质羰基含量；与对照组相比，DNA 碎片化和冷冻诱导的顶体反应变化。有趣的是，与其他组相比，II 组（10µM）中精子的早期凋亡样变化显著（p<.05）减少，同时具有高线粒体跨膜电位的精子细胞的高比例增加。仅在 II 组（10µM）中观察到较高的孕酮受体结合、先锋距离和体外获能反应。总之，在 Hariana 公牛精液 extender 中添加姜黄素具有抗冷冻保护作用，可在 10µM 浓度下添加到 extender 中以提高解冻后精子质量。
