Racioppo Angela, Speranza Barbara, Altieri Clelia, Sinigaglia Milena, Corbo Maria Rosaria, Bevilacqua Antonio
Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, Foggia, Italy.
Front Microbiol. 2023 Mar 6;14:1094671. doi: 10.3389/fmicb.2023.1094671. eCollection 2023.
The main goal of this research was to study the effect of an Ultrasound (US) treatment on biofilm formation of (strains c19 and DSM 1055), subsp. DSM 10140, subsp. DSM 20219, and subsp. DSM 20088. From a methodological point of view, each microorganism was treated through six US treatments, different for the power (10, 30, or 50% of the net power, 130 W), the duration (2, 6, or 10 min) and the application of pulses (0 or 10 s). After the treatment, a biofilm of the strains was let to form on glass slides and the concentration of sessile cells was analyzed for 16 days. Biofilms formed by untreated microorganisms were used as controls. As a first result, it was found that US significantly increased the concentration of sessile cells of subsp. , while for some other strains US treatment could not affect the formation of biofilm while improving its stability, as found for DSM1055 after 16 days. The variable mainly involved in this positive effect of US was the duration of the treatment, as biofilm formation and stability were improved only for 2 min-treatments; on the other hand, the effect of power and pulses were strain-dependent. In conclusion, the results suggest practical implication of a US pre-treatment for various fields (improvement of adhesion of microorganisms useful in food or in the gut, biomedical and environmental industries), although further investigations are required to elucidate the mode of action.
本研究的主要目的是研究超声（US）处理对嗜酸乳杆菌（菌株c19和DSM 1055）、德氏乳杆菌亚种保加利亚乳杆菌DSM 10140、德氏乳杆菌亚种乳酸乳杆菌DSM 20219和植物乳杆菌亚种DSM 20088生物膜形成的影响。从方法学角度来看，每种微生物都经过六次超声处理，处理的功率（净功率130 W的10%、30%或50%）、持续时间（2、6或10分钟）和脉冲应用（0或10秒）各不相同。处理后，让这些菌株的生物膜在载玻片上形成，并对固着细胞的浓度进行了16天的分析。未处理微生物形成的生物膜用作对照。作为第一个结果，发现超声显著增加了嗜酸乳杆菌亚种的固着细胞浓度，而对于其他一些菌株，超声处理虽然不能影响生物膜的形成，但能提高其稳定性，如16天后对DSM1055的观察结果。超声产生这种积极作用的主要变量是处理持续时间，因为仅2分钟的处理能改善生物膜的形成和稳定性；另一方面，功率和脉冲的影响因菌株而异。总之，这些结果表明超声预处理在各个领域（改善食品或肠道中有用微生物的粘附、生物医学和环境工业）具有实际意义，尽管还需要进一步研究来阐明其作用方式。
