缺氧诱导因子-1α和生存素在乳腺癌复发及预后中的作用
Role of hypoxia-inducible factor-1α and survivin in breast cancer recurrence and prognosis.
作者信息
Cao Qian, Mushajiang Munire, Tang Cheng-Qiong, Ai Xiu-Qing
机构信息
Department of Breast Radiotherapy, The Third Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Urumqi, 830011, Xinjiang, China.
Department of Radiological Physics and Technology, The Third Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Urumqi, 830011, Xinjiang, China.
出版信息
Heliyon. 2023 Mar 1;9(3):e14132. doi: 10.1016/j.heliyon.2023.e14132. eCollection 2023 Mar.
OBJECTIVE
To analyze the expression of hypoxia-inducible factor-1α (HIF-1α) and survivin in breast cancer, and different molecular subtypes of breast cancer and to assess their relationship with recurrence and prognosis.
METHODS
The expression levels of HIF-1α and survivin genes in breast cancer were investigated using bioinformatics. Their protein expression levels were then verified through immunohistochemistry (IHC), and their relationship with recurrence and prognosis was assessed.
RESULTS
Expression levels of HIF-1α and survivin genes and proteins were increased in breast cancer tissues compared with normal tissues. Both were associated with clinical features of breast cancer and differentially expressed in different molecular subtypes of breast cancer, and both are related to the signal pathway of breast cancer growth and invasion. HIF-1α and survivin gene and protein expression levels were correlated, and both were associated with breast cancer recurrence ( = 0.380, < 0.05; = 0.673, < 0.05, respectively). According to The Cancer Genome Atlas (TCGA) database, HIF1A and BIRC5 gene were not associated with breast cancer prognosis ( ≥ 0.05); however, HIF-1α and survivin protein were associated with recurrence patient's overall survival (OS) ( < 0.05).
CONCLUSION
HIF-1α and survivin are highly expressed in breast cancer and can be used as potential biomarkers to predict recurrence and assess prognosis.
目的
分析缺氧诱导因子-1α（HIF-1α）和生存素在乳腺癌及其不同分子亚型中的表达情况，并评估它们与复发和预后的关系。
方法
利用生物信息学研究乳腺癌中HIF-1α和生存素基因的表达水平。然后通过免疫组织化学（IHC）验证其蛋白表达水平，并评估它们与复发和预后的关系。
结果
与正常组织相比，乳腺癌组织中HIF-1α和生存素基因及蛋白的表达水平升高。两者均与乳腺癌的临床特征相关，在乳腺癌的不同分子亚型中差异表达，且均与乳腺癌生长和侵袭的信号通路有关。HIF-1α和生存素基因及蛋白表达水平相关，且均与乳腺癌复发相关（分别为r = 0.380，P < 0.05；r = 0.673，P < 0.05）。根据癌症基因组图谱（TCGA）数据库，HIF1A和BIRC5基因与乳腺癌预后无关（P≥0.05）；然而，HIF-1α和生存素蛋白与复发患者的总生存期（OS）相关（P < 0.05）。
结论
HIF-1α和生存素在乳腺癌中高表达，可作为预测复发和评估预后的潜在生物标志物。
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