Performance of mRNA as a biomarker for breast cancer among Vietnamese women.

OBJECTIVE

This study aimed to perform the reverse-transcription polymerase chain reaction (RT-PCR) to express the mRNA among patients with breast cancer in Vietnam and identify some potential associated clinical and pathological factors.

METHODS

Peripheral blood (PB) samples and tissues on 43 patients with breast cancer and 21 patients with fibroids were obtained. The Real-time RT-PCR and gene sequencing techniques were employed to detect survivin gene in breast cancer cell lines and cancer tissues.

RESULTS

mRNA transcription was detected in 32/43 (74,4%) of breast cancer tissues and 19/43 (44,2%) of PB samples of breast cancer patients, while it was detected in only 14,3 % fibrosis tissues and 0% in the blood of fibrosis patients. mRNA on the peripheral blood of breast cancer patients increased with tumor size, and stage of cancer (p < 0.05). In terms of breast cancer tissue, no difference was found in the rate of mRNA expression in according to age, distant metastasis, lymph node, stages of cancer, and histopathology (p > 0.05).

CONCLUSIONS

Results provide the initial evidence of the expression of survivin mRNA in breast cancer patients in Vietnam, suggesting the role of mRNA in breast cancer molecular pathology.