Nguyen Hien Minh, Dao Minh Quang, La Huyen Thi
Thanh Nhan Hospital, Hanoi, Viet Nam.
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam.
Heliyon. 2019 Mar 20;5(3):e01371. doi: 10.1016/j.heliyon.2019.e01371. eCollection 2019 Mar.
This study aimed to perform the reverse-transcription polymerase chain reaction (RT-PCR) to express the mRNA among patients with breast cancer in Vietnam and identify some potential associated clinical and pathological factors.
Peripheral blood (PB) samples and tissues on 43 patients with breast cancer and 21 patients with fibroids were obtained. The Real-time RT-PCR and gene sequencing techniques were employed to detect survivin gene in breast cancer cell lines and cancer tissues.
mRNA transcription was detected in 32/43 (74,4%) of breast cancer tissues and 19/43 (44,2%) of PB samples of breast cancer patients, while it was detected in only 14,3 % fibrosis tissues and 0% in the blood of fibrosis patients. mRNA on the peripheral blood of breast cancer patients increased with tumor size, and stage of cancer (p < 0.05). In terms of breast cancer tissue, no difference was found in the rate of mRNA expression in according to age, distant metastasis, lymph node, stages of cancer, and histopathology (p > 0.05).
Results provide the initial evidence of the expression of survivin mRNA in breast cancer patients in Vietnam, suggesting the role of mRNA in breast cancer molecular pathology.
本研究旨在通过逆转录聚合酶链反应（RT-PCR）检测越南乳腺癌患者的mRNA表达情况，并确定一些潜在的相关临床和病理因素。
采集43例乳腺癌患者和21例纤维瘤患者的外周血（PB）样本及组织。采用实时RT-PCR和基因测序技术检测乳腺癌细胞系和癌组织中的生存素基因。
在43例乳腺癌组织中的32例（74.4%）和乳腺癌患者PB样本中的19例（44.2%）检测到mRNA转录，而在纤维瘤组织中仅14.3%检测到，在纤维瘤患者血液中未检测到。乳腺癌患者外周血中的mRNA随肿瘤大小和癌症分期增加（p<0.05）。在乳腺癌组织方面，根据年龄、远处转移、淋巴结、癌症分期和组织病理学，mRNA表达率未发现差异（p>0.05）。
结果为越南乳腺癌患者生存素mRNA表达提供了初步证据，提示mRNA在乳腺癌分子病理学中的作用。
