operon heat shock-like regulation.

For decades, transcription of -operon was considered being constitutive. Therefore, this -operon has been used for measurements in non-specific bacterial luminescent biosensors. Here, the expression of -operon under high temperature was studied. The expression was researched in the natural strain and in the heterologous system of . FV2201 bacterium was isolated from soil in the Moscow region (growth optimum 28 °C). We showed that its luminescence significantly increases when the temperature rises to 34 °C. The increase in luminescence is associated with an increase in the transcription of genes, which was confirmed by RT-PCR. The promoter of the -operon of the related bacterium ZM1 from the forests of Moldova, being cloned in the heterologous system of , is activated when the temperature rises from room temperature to 42 °C. When heat shock is caused by ethanol addition, transcription of -operon increases only in the natural strain of , but not in the heterologous system of cells. In addition, the activation of the -operon of persists in strains deficient in both the and genes. These results indicate the presence of sigma 32 and sigma 24 independent heat-shock-like mechanism of regulation of the -operon of in the heterologous system.