Moscow Center for Advanced Studies, Moscow 123592, Russia.
A.N. Severtsov Institute of Ecology and Evolution of the RAS, Moscow 119071, Russia.
Biosensors (Basel). 2024 Sep 5;14(9):427. doi: 10.3390/bios14090427.
The ability of aquatic mesofauna representatives involved in trophic chains to sorb and accumulate toxicants is important for understanding the functioning of aquatic ecosystems and for fishing industry. This study investigated the capacity of marine amphipod and freshwater amphipods and to absorb the DNA-alkylating agent methyl methanesulfonate (MMS). The presence of alkylating agents in the environment and in the tissues of the amphipods was determined using whole-cell lux-biosensor MG1655 pAlkA-lux, in which the genes from , enabling the luminescence of the cell culture, are controlled by the P promoter of DNA glycosylase. It was shown that within one day of incubation in water containing MMS at a concentration above 10 μM, the amphipods absorbed the toxicant and their tissues produce more alkylation damage to biosensor cells than the surrounding water. Concentrations of MMS above 1 mM in the environment caused the death of the amphipods before the toxicant could be significantly concentrated in their tissues. The sensitivity and the capacity to absorb MMS were found to be approximately the same for the marine amphipod and the freshwater amphipods and .
参与食物链的水生中型动物代表具有吸附和积累毒物的能力，这对于了解水生生态系统的功能和渔业都很重要。本研究调查了海洋桡足类 和淡水桡足类 和 吸收 DNA 烷化剂甲磺酸甲酯 (MMS) 的能力。使用全细胞发光生物传感器 MG1655 pAlkA-lux 来确定环境中和桡足类组织中是否存在烷化剂，该传感器中包含来自 的 基因，这些基因受 DNA 糖苷酶的 P 启动子控制，使细胞培养发光。结果表明，在含有浓度高于 10 μM 的 MMS 的水中孵育一天后，桡足类吸收了有毒物质，其组织对生物传感器细胞造成的烷基化损伤比周围水更多。环境中 MMS 的浓度高于 1mM 会导致桡足类在有毒物质明显集中在其组织之前死亡。海洋桡足类 和淡水桡足类 和 对 MMS 的敏感性和吸收能力大致相同。
