Shi Xin, Yang Guang, Liu Ming-Yue, Yuan Meng-Tong, Wang Dong, Wang Xiao-Feng
Department of Prosthodontics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150000, PR China.
Department of Oral & Maxillofacial Surgery, The First Hospital of Qiqihar, Qiqihar, Heilongjiang Province, 161000, PR China.
Regen Med. 2023 Apr;18(4):313-327. doi: 10.2217/rme-2022-0206. Epub 2023 Mar 23.
To investigate the effect of hDPSC-Exos in flap I/R injury, a condition in which tissue damage increases after blood flow is restored to the flap after ischemia. HUVECs were used to investigate the influences and mechanisms of hDPSC-Exos on cell proliferation and migration. A rat model was established to verify the role of hDPSC-Exos in flap I/R injuries . hDPSC-Exos promoted the proliferation, migration and tube formation of HUVECs in a dose-dependent way by activating PI3K/AKT signaling pathway, and improved the survival and microvessel density of the flap and suppressed epithelial cell apoptosis. hDPSC-Exos can enhance flap repair after I/R injury. This process may be mediated by the activation of PI3K/AKT signaling pathway.
为研究人牙髓干细胞外泌体（hDPSC-Exos）在皮瓣缺血再灌注（I/R）损伤中的作用，皮瓣缺血再灌注损伤是指缺血后恢复皮瓣血流时组织损伤增加的一种情况。采用人脐静脉内皮细胞（HUVECs）研究hDPSC-Exos对细胞增殖和迁移的影响及机制。建立大鼠模型以验证hDPSC-Exos在皮瓣I/R损伤中的作用。hDPSC-Exos通过激活PI3K/AKT信号通路以剂量依赖的方式促进HUVECs的增殖、迁移和管腔形成，并改善皮瓣的存活和微血管密度，抑制上皮细胞凋亡。hDPSC-Exos可增强I/R损伤后皮瓣的修复。这一过程可能由PI3K/AKT信号通路的激活介导。
