Symbiosis Centre for Stem Cell Research, Symbiosis International (Deemed University), Pune, 412115, India.
Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune, 412115, India.
Regen Med. 2023 Apr;18(4):329-346. doi: 10.2217/rme-2022-0201. Epub 2023 Mar 23.
To explore the neuroprotective potential of the secretome (conditioned medium, CM) derived from neurotrophic factors-primed mesenchymal stromal cells (MSCs; primed CM) using an endoplasmic reticulum (ER) stress-induced model system. Establishment of ER-stressed model, immunofluorescence microscopy, real-time PCR, western blot. Exposure of ER-stressed Neuro-2a cells to the primed-CM significantly restored the neurite outgrowth parameters and improved the expression of neuronal markers like and in them compared with the naive CM. Primed CM also suppressed the induction of apoptotic markers and , inflammatory markers and NF-κB, and stress kinases such as p38 and SAPK/JNK in the stress-induced cells. The secretome from primed MSCs significantly restored ER stress-induced loss of neuro-regenesis.
探讨源自神经营养因子预刺激间充质基质细胞（primed MSCs；预刺激 CM）的分泌组（条件培养基）在内质网（ER）应激诱导模型系统中的神经保护潜能。
建立 ER 应激模型，免疫荧光显微镜检查，实时 PCR，western blot。
与对照 CM 相比，将 ER 应激的 Neuro-2a 细胞暴露于预刺激 CM 中可显著恢复其突起生长参数，并改善神经元标志物如 和 的表达。预刺激 CM 还抑制了应激诱导细胞中凋亡标志物 和 、炎症标志物 和 NF-κB 以及应激激酶如 p38 和 SAPK/JNK 的诱导。
预刺激 MSC 的分泌组可显著恢复 ER 应激诱导的神经再生丧失。
