Priming mesenchymal stromal cells with neurotrophic factors boosts the neuro-regenerative potential of their secretome.

To explore the neuroprotective potential of the secretome (conditioned medium, CM) derived from neurotrophic factors-primed mesenchymal stromal cells (MSCs; primed CM) using an endoplasmic reticulum (ER) stress-induced model system. Establishment of ER-stressed model, immunofluorescence microscopy, real-time PCR, western blot. Exposure of ER-stressed Neuro-2a cells to the primed-CM significantly restored the neurite outgrowth parameters and improved the expression of neuronal markers like and in them compared with the naive CM. Primed CM also suppressed the induction of apoptotic markers and , inflammatory markers and NF-κB, and stress kinases such as p38 and SAPK/JNK in the stress-induced cells. The secretome from primed MSCs significantly restored ER stress-induced loss of neuro-regenesis.