School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, P. R. China.
Department of Pharmacy, Ningbo City First Hospital, Ningbo, 315211, Zhejiang, P. R. China.
Analyst. 2023 Apr 11;148(8):1752-1763. doi: 10.1039/d3an00171g.
Owing to its promising biocompatibility and reliable sensitivity, semiconductor-guided surface-enhanced Raman scattering (SERS) technology has aroused widespread concern in clinical immunoassays. Herein, the well-improved light capture capability of MoS with a novel three-dimensional (3D) flower-like morphology was combined with the synergistic chemical enhancement from a MoS@red phosphorus (RP) hybrid system, facilitating an attractive non-metallic SERS-based detection of ferritin in serum. Owing to the remarkable enhancement factors of both the immunoprobe and immunosubstrate, which were comparable to noble metal, an extremely low limit detection of 11.5 pg mL was achieved in the absence of fluorescence interference. In particular, the trace ferritin in the clinical serum sample was successfully monitored, demonstrating superior sensitivity to the traditional chemiluminescent method. Overall, this study convincingly revealed the feasibility and reliability of SERS-based immunoassays induced by biocompatible semiconductors, which has opened a new way to implement the detection and tracking of biomarkers in the human body.
由于其 promising 的生物相容性和可靠的灵敏度，半导体引导的表面增强拉曼散射 (SERS) 技术在临床免疫分析中引起了广泛关注。在此，具有新颖的三维 (3D) 花状形态的 MoS 的改进的光捕获能力与 MoS@红磷 (RP) 混合系统的协同化学增强相结合，促进了血清中铁蛋白的有吸引力的非金属 SERS 检测。由于免疫探针和免疫底物的增强因子都非常显著，与贵金属相当，因此在没有荧光干扰的情况下，实现了极低的检测限 11.5 pg mL。特别是，成功监测到临床血清样本中的痕量铁蛋白，其灵敏度明显高于传统化学发光法。总的来说，本研究令人信服地揭示了基于生物相容性半导体的 SERS 免疫分析的可行性和可靠性，为在人体内实现生物标志物的检测和跟踪开辟了新途径。
