School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China.
Department of Pharmacy, Ningbo City First Hospital, Ningbo, 315211, Zhejiang, PR China.
Colloids Surf B Biointerfaces. 2023 Jul;227:113338. doi: 10.1016/j.colsurfb.2023.113338. Epub 2023 May 8.
To improve the curability of cancer patients, it is essential to propose an early diagnosis technology with ultra-high sensitivity and reliable biocompatibility. Herein, a sophisticated nonmetallic SERS-based immunosensor, comprised by a MoS @FeO nanoflower-based immunoprobe with magnetism and a black phosphorus (BP) nanosheet-based immunosubstrate, was proposed for the specific in-situ monitoring of ferritin (FER). The sandwich immunosensor was endowed with an excellent SERS performance mainly ascribed to a synergistic chemical enhancement as well as an additional electrostatic adsorption effect, achieving a limit of detection down to 7.3 × 10 μg/mL. Particularly, all the Raman label, target FER, and anti-FER could be completely degraded within 70 min under visible light irradiation owing to the favorable photocatalytic activities of MoS and BP which could be then effectively separated and collected with the assistance of an external magnet. Such a recyclable nonmetallic immunosensor holds great potential and practicality in the clinical screening of cancer.
为提高癌症患者的治愈率，提出一种超灵敏、可靠生物相容性的早期诊断技术至关重要。在此，我们提出了一种复杂的基于无金属 SERS 的免疫传感器，由具有磁性的 MoS@FeO 纳米花基免疫探针和基于黑磷（BP）纳米片的免疫基底组成，用于铁蛋白（FER）的特异性原位监测。三明治免疫传感器具有优异的 SERS 性能，主要归因于协同化学增强以及额外的静电吸附效应，检测限低至 7.3×10μg/mL。特别地，由于 MoS 和 BP 的光催化活性良好，所有的拉曼标记物、目标 FER 和抗-FER 在可见光照射下 70 分钟内可完全降解，然后在外磁场的帮助下可有效分离和收集。这种可回收的非金属免疫传感器在癌症的临床筛查中具有很大的潜力和实用性。
