Kowalski S R, Gourlay G K, Cherry D A, McLean C F
Pain Management Unit, Flinders Medical Centre, Bedford Park, South Australia.
J Pharmacol Methods. 1987 Dec;18(4):347-55. doi: 10.1016/0160-5402(87)90066-0.
A method for the determination of fentanyl blood concentrations using gas liquid chromatography coupled to a nitrogen phosphorus detector (NPD) is presented. A highly inert fused-silica, megabore column coated with a methyl silicone stationary phase was used for the analysis. The mean coefficient of variation for the range of fentanyl concentrations tested (0.25-10 ng/ml) was 4.65%, ranging from 0.85% at 10 ng/ml to 10.8% at 0.25 ng/ml. The assay was used to quantify blood fentanyl concentrations collected from a 56-year-old woman who was administered fentanyl postoperatively via a patient-controlled on-demand analgesic computer (ODAC). The mean hourly fentanyl dose rate over the 44 hr study period was 41.8 micrograms/hr (range 20-120). The sixfold variation in hourly dose rate was not mirrored by similar fluctuations in the fentanyl blood concentration (mean 0.45 ng/ml, range 0.3-0.7 ng/ml). The patient thus titrated herself to a perceived minimum effective concentration (MEC) of fentanyl.
介绍了一种使用气相色谱-氮磷检测器（NPD）测定芬太尼血药浓度的方法。分析采用了一根涂覆甲基硅氧烷固定相的高惰性熔融石英大口径柱。所测试的芬太尼浓度范围（0.25 - 10 ng/ml）的平均变异系数为4.65%，从10 ng/ml时的0.85%到0.25 ng/ml时的10.8%。该检测方法用于对一名56岁女性术后通过患者自控按需镇痛计算机（ODAC）给予芬太尼后采集的血样中芬太尼浓度进行定量。在44小时的研究期间，芬太尼的平均每小时剂量率为41.8微克/小时（范围为20 - 120）。每小时剂量率的六倍变化并未反映在芬太尼血药浓度的类似波动中（平均0.45 ng/ml，范围0.3 - 0.7 ng/ml）。因此，该患者自行滴定至其感知到的芬太尼最低有效浓度（MEC）。
