Institute of Biology, University of Szczecin, 71-412 Szczecin, Poland.
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin,1 Chodźki Str., 20-093 Lublin, Poland.
Infect Genet Evol. 2023 Jun;110:105427. doi: 10.1016/j.meegid.2023.105427. Epub 2023 Mar 21.
Lagovirus europaeus/GI.1 is the virus that causes severe and dangerous rabbit haemorrhagic disease (RHD) in rabbits. Recombination formation in RHD viruses is common. Recombination is thought to play a key role in the evolution of lagoviruses and therefore most likely influences the pathogenicity of L. europaeus/GI strains. Immunological events also play a key role in the control of RHD, and an in-depth knowledge of these phenomena provides insights into the characteristics of the infection, which can help implement appropriate infection control measures. To obtain a more complete picture of RHD caused by different GI.1 strains, it is necessary to correlate the genetic diversity within L. europaeus/GI.1 strains and the immune picture in response to infection. We performed a phylogenetic analysis of the L. europaeus/GI strains and compared the recombinant L. europaeus/GI.1 strain with the GI.1a strain on the basis of a thorough statistical analysis of immunological traits performed previously. Our phylogenetic analysis based on the sequence of the gene encoding the VP60 capsid protein of 34 strains of Lagovirus europaeus showed that the Hartmannsdorf strain forms a separate clade from the other GI.1a strains and is separate from the GI.1b-d strains. Next, we showed significant differences in the levels of individual parameters for non-specific cellular and humoral immunity in infection with the GI.1a strain and the Hartmannsdorf recombinant strain. Against the background of this study, our results indicate that the characteristics of each recombinant should be considered individually.
兔出血症病毒（RHDV）是引起家兔严重且危险的兔出血症（RHD）的病毒。RHD 病毒的重组形成很常见。重组被认为在长尾病毒的进化中起着关键作用，因此极有可能影响 L. europaeus/GI 株的致病性。免疫事件也在 RHD 的控制中起着关键作用，深入了解这些现象可以深入了解感染的特征，从而有助于实施适当的感染控制措施。为了更全面地了解不同 GI.1 株引起的 RHD，有必要将 L. europaeus/GI.1 株内的遗传多样性与感染后的免疫情况相关联。我们对 L. europaeus/GI 株进行了系统发育分析，并在先前对免疫特性进行了彻底的统计分析的基础上，比较了重组 L. europaeus/GI.1 株与 GI.1a 株。我们基于编码 34 株 L. europaeus 病毒 VP60 衣壳蛋白的基因序列进行的系统发育分析表明，Hartmannsdorf 株与其他 GI.1a 株形成一个独立的分支，与 GI.1b-d 株分开。接下来，我们表明，在感染 GI.1a 株和 Hartmannsdorf 重组株时，非特异性细胞和体液免疫的个体参数水平存在显著差异。在本研究的背景下，我们的研究结果表明，每个重组株的特征都应单独考虑。
