杏仁核（西伯利亚杏）中性多糖的结构表征、α-葡萄糖苷酶抑制活性和抗氧化活性。
Structural characterization, α-glucosidase inhibitory activity and antioxidant activity of neutral polysaccharide from apricot (Armeniaca Sibirica L. Lam) kernels.
机构信息
College of Food Science, Shenyang Agricultural University, Shenyang 11086, China.
College of Food Science, Shenyang Agricultural University, Shenyang 11086, China.
出版信息
Int J Biol Macromol. 2023 May 31;238:124109. doi: 10.1016/j.ijbiomac.2023.124109. Epub 2023 Mar 21.
Screening for α-glucosidase inhibitors and antioxidants from natural sources that could reduce postprandial glucose in diabetic patients and reduce oxidative stress had attracted considerable interest. In this study, a neutral polysaccharide (AP-1) with a triple helix structure was isolated and purified from the residue of apricot (Armeniaca sibirica L. Lam.) kernels by using DEAE-52 and Sephadex G-100 columns. The molecular weight of AP-1 was 23.408 kDa and consisted mainly of glucose with trace amounts of arabinose, galactose, and mannose, which had molar percentages of 98.48, 0.63, 0.62 and 0.27 %, respectively. The main chain of AP-1 was composed of →4)-α-D-Glcp-(1 → interlinked, and α-D-Glcp-(1 → was attached as a branched chain at the O-6 position of →4,6)-α-D-Glcp-(1→. In addition, AP-1 exhibited stronger α-glucosidase inhibition and free radical scavenging ability compared to crude polysaccharides. Therefore, AP-1 could be used as a potential natural hypoglycemic agent and antioxidant in the treatment of diabetes mellitus.
从天然来源筛选能够降低糖尿病患者餐后血糖和降低氧化应激的α-葡萄糖苷酶抑制剂和抗氧化剂引起了相当大的兴趣。在这项研究中，从杏仁（西伯利亚杏）核废渣中分离并纯化出一种具有三螺旋结构的中性多糖（AP-1），使用 DEAE-52 和 Sephadex G-100 柱。AP-1 的分子量为 23.408 kDa，主要由葡萄糖组成，含有微量的阿拉伯糖、半乳糖和甘露糖，摩尔百分比分别为 98.48%、0.63%、0.62%和 0.27%。AP-1 的主链由→4)-α-D-Glcp-(1→相互连接，α-D-Glcp-(1→作为支链连接在→4,6)-α-D-Glcp-(1→的 O-6 位。此外，AP-1 表现出比粗多糖更强的α-葡萄糖苷酶抑制和自由基清除能力。因此，AP-1 可用作治疗糖尿病的潜在天然降血糖剂和抗氧化剂。
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