College of Life Science, Fujian Normal University, Fuzhou 350117, China.
College of Life Science, Fujian Normal University, Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350117, China.
Bioresour Technol. 2023 Jun;377:128945. doi: 10.1016/j.biortech.2023.128945. Epub 2023 Mar 21.
This work was the first time to systematically clarify the potential tolerance mechanism of an indigenous Chlorella vulgaris MBFJNU-1 towards the free ammonia (FA) during the original swine wastewater (OSW) treatment by transcriptome analysis using C. vulgaris UETX395 as the control group. The obtained results showed that C. vulgaris MBFJNU-1 was found to be more resistant to the high levels of FA (115 mg/L) and OSW in comparison to C. vulgaris UETX395 (38 mg/L). Moreover, the transcriptomic results stated that some key pathways from arginine biosynthesis, electron generation and transmission, ATP synthesis in chloroplasts, and glutathione synthesis of C. vulgaris MBFJNU-1 were greatly related with the OSW and FA. Additionally, C. vulgaris MBFJNU-1 in OSW and FA performed similar results in the common differentially expressed genes from these mentioned pathways. Overall, these obtained results deliver essential details in microalgal biotechnology to treat swine wastewater and high free ammonia wastewater.
本研究首次通过转录组分析，利用普通小球藻 UETX395 作为对照组，系统阐明了土著小球藻 MBFJNU-1 在处理原猪废水（OSW）过程中对游离氨（FA）的潜在耐受机制。研究结果表明，与普通小球藻 UETX395（38mg/L）相比，小球藻 MBFJNU-1 对高浓度 FA（115mg/L）和 OSW 更具抗性。此外，转录组结果表明，小球藻 MBFJNU-1 中精氨酸生物合成、电子产生和传递、叶绿体中 ATP 合成以及谷胱甘肽合成等关键途径与 OSW 和 FA 密切相关。此外，小球藻 MBFJNU-1 在 OSW 和 FA 中，这些途径中的共同差异表达基因表现出相似的结果。总的来说，这些研究结果为利用微藻生物技术处理猪废水和高游离氨废水提供了重要信息。
