从四位患者的纵向样本中分离猴痘病毒以推断传播风险：一项中期分析。
Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis.
机构信息
Rare and Imported Pathogens Laboratory, UKHSA, Porton Down, UK; Aberdeen Royal Infirmary, Aberdeen, UK.
High Containment & Biological Services, Salisbury, UK.
出版信息
J Hosp Infect. 2023 May;135:139-144. doi: 10.1016/j.jhin.2023.03.009. Epub 2023 Mar 21.
Abstract
Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.
摘要
猴痘病毒（MPXV）是正痘病毒属中的一种 DNA 病毒，可导致猴痘（以前称为猴痘）。症状包括发热、淋巴结病和水疱性病变。关于 MPXV 的感染性持续时间的证据有限。本研究使用细胞培养作为感染性的替代指标。将来自 4 名猴痘患者的临床样本接种到非洲绿猴肾（Vero E6）细胞中，并监测细胞病变效应（CPE）。从一名患者中，在发病后 25 天恢复了具有感染性的 MPXV。从聚合酶链反应（PCR）Ct 值超过 31.0 的样本或尿液中均未分离到感染性病毒。
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