从韩国首例病例中分离并鉴定猴痘病毒MPXV-ROK-P1-2022
Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea.
作者信息
Kim Jin-Won, Lee Minji, Shin Hwachul, Choi Chi-Hwan, Choi Myung-Min, Kim Jee Woong, Yi Hwajung, Yoo Cheon-Kwon, Rhie Gi-Eun
机构信息
Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea.
Division of Research Support, Department of Research Planning and Coordination, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, Korea.
出版信息
Osong Public Health Res Perspect. 2022 Aug;13(4):308-311. doi: 10.24171/j.phrp.2022.0232. Epub 2022 Aug 31.
OBJECTIVES
Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient's specimens.
METHODS
Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products.
RESULTS
Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing.
CONCLUSION
These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.
目的
自2022年5月初以来，已有非猴痘流行国家报告了猴痘疫情。韩国首例猴痘病例于2022年6月在一名前往欧洲旅行的患者中得到确诊，并尝试从患者标本中分离和鉴定猴痘病毒（MPXV）。
方法
将患者的临床标本接种于Vero E6细胞。通过观察Vero E6细胞上的细胞病变效应、透射电子显微镜、常规聚合酶链反应（PCR）以及PCR产物测序，将分离出的病毒鉴定为MPXV。
结果
在用皮肤病变拭子洗脱液接种的Vero E6细胞中观察到细胞病变效应。从原代培养物多次传代后，使用透射电子显微镜观察到正痘病毒形态。此外，通过常规PCR和桑格测序确认了MPXV特异性基因（F3L和ATI）以及正痘病毒特异性基因（A39R、B2R和HA）。
结论
这些结果表明已成功从韩国首例患者中分离并鉴定出MPXV。分离出的病毒被命名为MPXV-ROK-P1-2022。
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