Department of Breast Surgery, Nanfang Hospital, Southern Medical University, Guangzhou City, China.
Department of Clinical Nutrition, Southern Theater Air Force Hospital, Guangzhou City, China.
Thorac Cancer. 2023 May;14(13):1135-1144. doi: 10.1111/1759-7714.14834. Epub 2023 Mar 23.
Sauchinone is extracted from the root of Saururus chinensis and exhibits potent antitumor effects in various human cancers. However, how sauchinone is involved in breast cancer has not been well studied.
Cells apoptosis, cell proliferation, and cycle distribution were evaluated. Xenograft tumor mouse model was constructed to investigate the roles of sauchinone. The relevant protein expression was detected by western blot.
We found that sauchinone significantly reduced proliferation and survival, also induced apoptosis of MCF-7 and Bcap-37 cells in vitro. Sauchinone significantly increased miR-148a-3p expression, and human epidermal growth factor receptor (HER)-2 targeted on miR-148a-3p. Sauchinone exposure downregulated HER-2 expression whose overexpression partly eliminated the inhibitory effect of sauchinone. Further, sauchinone efficiently inhibited breast cancer progression through downregulating HER-2 expression in vivo.
Our results indicate that sauchinone efficiently inhibits breast cancer progression through regulating miR-148a-3p/HER-2 axis, suggesting that sauchinone could be an effective anticancer agent for breast cancer.
沙蟾酮从中华石龙子的根中提取，对多种人类癌症具有强大的抗肿瘤作用。然而，沙蟾酮如何参与乳腺癌的发生还没有得到很好的研究。
评估细胞凋亡、细胞增殖和细胞周期分布。构建异种移植肿瘤小鼠模型，研究沙蟾酮的作用。通过 Western blot 检测相关蛋白表达。
我们发现沙蟾酮显著降低 MCF-7 和 Bcap-37 细胞的增殖和存活，体外诱导细胞凋亡。沙蟾酮显著增加 miR-148a-3p 的表达，而人类表皮生长因子受体（HER）-2 靶向 miR-148a-3p。沙蟾酮暴露下调 HER-2 表达，其过表达部分消除了沙蟾酮的抑制作用。此外，沙蟾酮通过下调体内 HER-2 表达有效地抑制了乳腺癌的进展。
我们的研究结果表明，沙蟾酮通过调节 miR-148a-3p/HER-2 轴有效地抑制乳腺癌的进展，提示沙蟾酮可能是一种有效的乳腺癌抗癌药物。
