Department of Animal Physiology, University of Bayreuth, (Universitätsstraße 30, D-95440 Bayreuth), Bayreuth, Germany.
Commun Biol. 2023 Mar 23;6(1):309. doi: 10.1038/s42003-023-04695-4.
Although isoeugenol is one of the most widely used anesthetics in fish, its actual mode of action and thus its applicability for particular interventions is poorly understood. Here we determined effects of isoeugenol on various aspects of sensory and neural function, taking advantage of intracellular in vivo recordings in a uniquely suited identified neuron, the Mauthner neuron in the brain of goldfish. We show that isoeugenol strongly affects hearing and vision, but sensitivity and time course of action differed largely in these two senses. The action potential, chemical and electric synaptic transmission at the central neuron were not affected at low but efficient anesthesia. Effects seen at high concentration thereby do not support current views of how isoeugenol might act on central neurons. We show that isoeugenol is highly useful to anesthetize fish for handling, but that in more severe treatment its application needs to be carefully adapted to task.
尽管异丁香酚是鱼类中应用最广泛的麻醉剂之一，但人们对其实际作用机制及其在特定干预措施中的适用性知之甚少。在这里，我们利用金鱼大脑中一种独特的、已鉴定神经元——Mauthner 神经元的细胞内体内记录，确定了异丁香酚对感觉和神经功能各个方面的影响。我们发现异丁香酚对听觉和视觉有强烈影响，但在这两种感觉中，其作用的敏感性和时程有很大差异。在低浓度下，动作电位、化学和电突触传递在中枢神经元中不受影响，但在高效麻醉时则受到影响。在高浓度下观察到的作用并不支持目前关于异丁香酚如何作用于中枢神经元的观点。我们表明，异丁香酚非常适合用于麻醉鱼类以进行处理，但在更严重的治疗中，需要根据任务仔细调整其应用。
