Mielczarek Przemyslaw, Suder Piotr, Kotsan Igor, Bodzon-Kulakowska Anna
Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smetna 12 St., 31-343, Krakow, Poland.
Department of Analytical Chemistry and Biochemistry, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, A. Mickiewicza 30 Ave., 30-059, Krakow, Poland.
J Mass Spectrom. 2023 Mar 16;58(4):e4916. doi: 10.1002/jms.4916.
Imaging mass spectrometry is a powerful technique for the molecular analysis of tissue sections. As in many analytical methods, sample preparation is one of the main and most important steps to obtain results of good quality. Usually, the matrix concentration and solvent composition in different studies are taken for granted without any further consideration. In our studies, we aimed to find how matrix concentration and a type of solvent influence the signal. Moreover, we also aimed to find the relationship between these parameters, how they influence the spectra, and how they influence obtained ion maps. In our experiments, we used SunCollect®, which is a commercially available wet-interface system for matrix deposition. We decided to choose two matrix concentrations (2,5-dihydroxybenzoic acid [DHB]: 15 and 25 mg/mL; 9-aminoacridine [9AA]: 7 and 5 mg/mL) and two different water solutions of solvents in two different percentages for the matrices (DHB: 50% and 70% of methanol [MeOH] and acetonitrile [ACN]; 9AA 70% and 50% of ethanol [EtOH] and MeOH). In the end, the influence of these parameters on obtained spectra and ion maps was assessed.
成像质谱是一种用于组织切片分子分析的强大技术。与许多分析方法一样，样品制备是获得高质量结果的主要且最重要的步骤之一。通常，不同研究中的基质浓度和溶剂组成被视为理所当然，没有进一步考虑。在我们的研究中，我们旨在探究基质浓度和溶剂类型如何影响信号。此外，我们还旨在找出这些参数之间的关系、它们如何影响光谱以及如何影响所获得的离子图像。在我们的实验中，我们使用了SunCollect®，它是一种用于基质沉积的市售湿界面系统。我们决定选择两种基质浓度（2,5 - 二羟基苯甲酸 [DHB]：15和25 mg/mL；9 - 氨基吖啶 [9AA]：7和5 mg/mL）以及两种不同百分比的两种不同溶剂水溶液用于基质（DHB：50%和70%的甲醇 [MeOH] 和乙腈 [ACN]；9AA：70%和50%的乙醇 [EtOH] 和MeOH）。最后，评估了这些参数对所获得的光谱和离子图像的影响。
