Hua Tebo, Yang Jiahui, Zhu Ye, Luo Yong
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.
Department of Thyroid Breast Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo, Zhejiang 315040, P.R. China.
Oncol Lett. 2023 Mar 8;25(4):165. doi: 10.3892/ol.2023.13752. eCollection 2023 Apr.
Long non-coding RNA Down syndrome cell adhesion molecule-antisense 1 (DSCAM-AS1) has been reported to play key roles in the progression and initiation of several cancer types. However, the various functional roles of DSCAM-AS1 in thyroid cancer tumorigenesis remain largely elusive. In the present study, the expression of DSCAM-AS1 was examined in thyroid cancer tissues and cell lines. Cell Counting Kit-8, wound healing, Transwell and clonogenic assays were conducted to detect cell proliferation, migration, invasion and colony formation, respectively. The association of DSCAM-AS1 with microRNA 211 (miR-211) was determined by luciferase reporter assay. It was found that the expression of DSCAM-AS1 was upregulated in thyroid cancer cells and tissues. Furthermore, enhanced DSCAM-AS1 expression was positively associated with lymph node metastasis and tumor-node-metastasis stage. Functional experiments demonstrated that DSCAM-AS1 knockdown inhibited the migration, proliferation and invasion of TPC-1 cells. Mechanistically, DSCAM-AS1 could bind to miR-211. Prevention of miR-211 by a miR-211 inhibitor reversed the effect of DSCAM-AS1 depletion in thyroid cancer tumorigenesis. Briefly, the current findings suggested that knockdown of DSCAM-AS1 suppressed the tumorigenesis of thyroid cancer via regulating miR-211, suggesting that DSCAM-AS1 may be a favorable therapeutic target for thyroid cancer.
据报道，长链非编码RNA唐氏综合征细胞粘附分子反义1（DSCAM-AS1）在多种癌症类型的进展和发生中起关键作用。然而，DSCAM-AS1在甲状腺癌发生中的各种功能作用仍不清楚。在本研究中，检测了DSCAM-AS1在甲状腺癌组织和细胞系中的表达。分别进行细胞计数试剂盒-8、伤口愈合、Transwell和克隆形成试验，以检测细胞增殖、迁移、侵袭和集落形成。通过荧光素酶报告基因试验确定DSCAM-AS1与微小RNA 211（miR-211）的关联。研究发现，DSCAM-AS1在甲状腺癌细胞和组织中的表达上调。此外，DSCAM-AS1表达增强与淋巴结转移和肿瘤-淋巴结-转移分期呈正相关。功能实验表明，敲低DSCAM-AS1可抑制TPC-1细胞的迁移、增殖和侵袭。机制上，DSCAM-AS1可与miR-211结合。用miR-211抑制剂阻断miR-211可逆转DSCAM-AS1缺失对甲状腺癌发生的影响。简而言之，目前的研究结果表明，敲低DSCAM-AS1通过调节miR-211抑制甲状腺癌的发生，提示DSCAM-AS1可能是甲状腺癌的一个良好治疗靶点。
