Zeng Qingchao, Man Xiaowu, Huang Zeyang, Zhuang Lubo, Yang Hanmeng, Sha Yuexia
Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.
Front Microbiol. 2023 Mar 7;14:1129614. doi: 10.3389/fmicb.2023.1129614. eCollection 2023.
Soil salinity is a prevalent environmental stress in agricultural production. Microbial inoculants could effectively help plants to alleviate salt stress. However, there is little knowledge of the biocontrol strain Ej2 mechanisms aiding rice plants to reduce the adverse effects caused by salt stress.
We performed integrated field and greenhouse experiments, microbial community profiling, and rice proteomic analysis to systematically investigate the Ej2 mechanism of action.
The results displayed that biocontrol strain Ej2 increased shoot/root length and fresh/dry weight compared with control under salt stress. Meanwhile, strain Ej2 has the ability to control rice blast disease and promote rice growth. Furthermore, the microbial community analysis revealed that the alpha-diversity of Ej2-inoculated plants was higher than the control plants, expect the Shannon index of the bacterial microbiome and the Ej2-inoculated samples clustered and separated from the control samples based on beta-diversity analysis. Importantly, the enriched and specific OTUs after Ej2 inoculation at the genus level were , , , and . Moreover, we observed that Ej2 inoculation influenced the rice proteomic profile, including metabolism, plant-pathogen interactions, and biosynthesis of unsaturated fatty acids. These results provide comprehensive evidence that Ej2 inoculation induced the rice endophytic microbiome and proteomic profiles to promote plant growth under salt stress.
Understanding the biocontrol strain effects on the endophytic microbiome and rice proteomics will help us better understand the complex interactions between plants and microorganisms under salt stress. Furthermore, unraveling the mechanisms underlying salt tolerance will help us more efficiently ameliorate saline soils.
土壤盐渍化是农业生产中普遍存在的环境胁迫。微生物接种剂可以有效帮助植物缓解盐胁迫。然而，对于生防菌株Ej2帮助水稻植株减轻盐胁迫造成的不利影响的机制知之甚少。
我们进行了田间和温室综合试验、微生物群落分析以及水稻蛋白质组分析，以系统地研究Ej2的作用机制。
结果显示，在盐胁迫下，与对照相比，生防菌株Ej2增加了地上部/根长以及鲜重/干重。同时，菌株Ej2具有防治稻瘟病和促进水稻生长的能力。此外，微生物群落分析表明，接种Ej2的植株的α多样性高于对照植株，除了细菌微生物组的香农指数，并且基于β多样性分析，接种Ej2的样品与对照样品聚类并分离。重要的是，Ej2接种后在属水平上富集的特定操作分类单元有、、和。此外，我们观察到Ej2接种影响了水稻蛋白质组图谱，包括代谢、植物-病原体相互作用以及不饱和脂肪酸的生物合成。这些结果提供了全面的证据，表明接种Ej2诱导了水稻内生微生物组和蛋白质组图谱，以促进盐胁迫下的植物生长。
了解生防菌株对内生微生物组和水稻蛋白质组学的影响将有助于我们更好地理解盐胁迫下植物与微生物之间的复杂相互作用。此外，揭示耐盐机制将有助于我们更有效地改良盐渍土壤。
