Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, CZ-62500, Czech Republic.
Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic.
J Biomed Mater Res A. 2023 Sep;111(9):1333-1343. doi: 10.1002/jbm.a.37531. Epub 2023 Mar 24.
As the consumption of implants increases, so do the requirements for individual types of implants, for example, improved biocompatibility or longevity. Therefore, the nano-modification of the titanium surface is often chosen. The aim was to characterize the modified surface with a focus on medical applications. The titanium surface was modified by the anodic oxidation method to form nanotubes. Subsequently, the material was characterized and analyzed for medical applications-surface morphology, surface wettability, chemical composition, and release of ions into biological fluids. A human gingival fibroblasts (HGFb) cell line was used in the viability study. A homogeneous layer of nanotubes of defined dimensions was formed on the titanium surface, ensuring the material's biocompatibility-the preparation conditions influence the resulting properties of the nanostructured surface. Nanostructured titanium exhibited more suitable characteristics (e.g., wettability, roughness, ion release) for biological applications than compared to pure titanium. It was possible to understand the behavior of the modified layer on the titanium surface and its effect on cell behavior. Another contribution of this work is the combination of material characterization (ion release) with the study of cytocompatibility (direct contact of cells with metals).
随着植入物的消费增加，对各种类型植入物的要求也越来越高，例如，提高生物相容性或延长使用寿命。因此，经常选择对钛表面进行纳米改性。目的是用重点关注医疗应用的方法来对改性表面进行特征描述。通过阳极氧化法对钛表面进行改性以形成纳米管。随后，对材料进行了表面形貌、表面润湿性、化学成分以及向生物流体中释放离子的医学应用分析和表征。在生存能力研究中使用了人牙龈成纤维细胞（HGFb）细胞系。在钛表面上形成了一层均匀的、具有确定尺寸的纳米管，从而确保了材料的生物相容性-制备条件会影响纳米结构表面的最终性能。与纯钛相比，纳米结构钛表现出更适合生物应用的特性（例如，润湿性、粗糙度、离子释放）。可以理解改性层在钛表面上的行为及其对细胞行为的影响。这项工作的另一个贡献是将材料特性（离子释放）与细胞相容性研究（细胞与金属的直接接触）相结合。
