Department of Child Health, Faculty of Medicine, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, Indonesia.
Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Ann Med. 2023 Dec;55(1):1211-1215. doi: 10.1080/07853890.2023.2190162.
Type 1 diabetes mellitus (T1DM) is disease caused by the destruction of β pancreatic cells. The activation of T-lymphocyte and proliferation inhibitor are induced by protein tyrosine phosphatase non-receptor type 22 (). However, the link between C1858T gene polymorphism and T1DM is still controversy. This study aimed to analyse the C1858T gene polymorphism in Indonesian children with T1DM.
This case-control study was conducted from March 2021 to May 2022 in the Endocrinology Outpatient Clinic at Dr. Soetomo Hospital and Tropical Disease Center Universitas Airlangga. Patients with controlled T1DM during the study period were included. The analysis used polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method.
Sixty-two children voluntarily participated in this study, and were equally divided into the T1DM and control groups. Most of the patients (94%, 58/62) are Javanese. This study revealed a more frequent CC genotype (9.4%) and allele-C (54.6%) polymorphism in the T1DM group, while more frequent CT genotype (100%) and allele-T (50%) polymorphism were in the control group. The C- and T-allele frequency was 54.6% and 45.4% in the T1DM group, respectively. The T1DM and control groups did not significantly differ (= .2381).
homozygous genotype-CC and allele-C polymorphisms are more frequent in patients with T1DM. However, the C1858T gene polymorphism did not significantly correlate to T1DM children in this study.Key Messages:The C1858T gene polymorphism does not significantly affect the susceptibility of T1DM in Indonesian children. homozygous genotype-CC polymorphism was more observed in the T1DM group; thus, this genotype may play as a risk factor for T1DM children in the Indonesian population.
1 型糖尿病（T1DM）是由β胰岛细胞破坏引起的疾病。蛋白酪氨酸磷酸酶非受体型 22（）的 T 淋巴细胞激活和增殖抑制剂被诱导。然而，C1858T 基因多态性与 T1DM 之间的联系仍存在争议。本研究旨在分析印度尼西亚 T1DM 患儿的 C1858T 基因多态性。
本病例对照研究于 2021 年 3 月至 2022 年 5 月在苏加诺医院内分泌科和阿里朗加大学热带病中心进行。纳入研究期间血糖控制良好的 T1DM 患者。采用聚合酶链反应限制片段长度多态性（PCR-RFLP）法进行分析。
62 名儿童自愿参与本研究，平均分为 T1DM 组和对照组。大多数患者（94%，58/62）为爪哇人。本研究显示 T1DM 组 CC 基因型（9.4%）和 C 等位基因（54.6%）的多态性更为常见，而对照组 CT 基因型（100%）和 T 等位基因（50%）的多态性更为常见。T1DM 组 C 和 T 等位基因频率分别为 54.6%和 45.4%。T1DM 组和对照组之间无显著差异（= 0.2381）。
T1DM 患者纯合基因型-CC 和等位基因-C 多态性更为常见。然而，本研究中 C1858T 基因多态性与 T1DM 儿童无显著相关性。关键信息：C1858T 基因多态性对印度尼西亚儿童 1 型糖尿病的易感性无显著影响。在 T1DM 组中观察到更多的纯合基因型-CC 多态性；因此，这种基因型可能是印度尼西亚人群中 T1DM 儿童的一个危险因素。
