Zhao Kai, Han Dong, He Si-Rui, Wu Long-Yan, Liu Wu-Yang, Zhong Zhao-Ming
Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China; Department of Orthopaedics, First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, PR China.
Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China.
Biochem Biophys Res Commun. 2023 May 14;656:115-121. doi: 10.1016/j.bbrc.2023.03.045. Epub 2023 Mar 17.
Bone marrow endothelial cells (BMECs) play a crucial role in the maintenance of bone homeostasis. The decline in BMECs is associated with abnormal bone development and loss. At present, the mechanism of age-related oxidative stress enhancement in BMEC dysfunction remains unclear. Our experiment explored injury caused by oxidative stress enhancement in BMECs both in vivo and in vitro. The BMECs, indicators of oxidative stress, bone mass, and apoptosis-related proteins were analyzed in different age groups. We also evaluated the ability of N-Acetyl-L-cysteine (NAC) attenuate oxidative stress injury in BMECs. NAC treatment attenuated reactive oxygen species (ROS) overgeneration and apoptosis in BMECs in vitro and alleviated the loss of BMECs and bone mass in vivo. In conclusion, this study could improve our understanding of the mechanism of oxidative stress-induced BMECs injury and whether NAC has therapeutic potential in senile osteoporosis.
骨髓内皮细胞（BMECs）在维持骨稳态中起关键作用。BMECs数量的减少与骨骼发育异常和骨量丢失有关。目前，BMEC功能障碍中与年龄相关的氧化应激增强的机制尚不清楚。我们的实验在体内和体外探究了氧化应激增强对BMECs造成的损伤。分析了不同年龄组中BMECs、氧化应激指标、骨量及凋亡相关蛋白。我们还评估了N-乙酰半胱氨酸（NAC）减轻BMECs氧化应激损伤的能力。NAC处理可减轻体外培养的BMECs中活性氧（ROS）的过度生成和细胞凋亡，并减轻体内BMECs丢失和骨量减少的情况。总之，本研究有助于我们更好地理解氧化应激诱导BMECs损伤的机制，以及NAC在老年性骨质疏松症中是否具有治疗潜力。
