Ichikawa T, Ishida I, Hirata Y, Deguchi T
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neurosciences, Japan.
Neurosci Lett. 1987 Oct 16;81(1-2):24-8. doi: 10.1016/0304-3940(87)90334-x.
Two monoclonal antibodies to monkey brain choline acetyltransferase (ChAT) were obtained. Immunoblot analysis indicated that both antibodies revealed two adjacent protein bands on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ChAT was demonstrated immunohistochemically by one of the antibodies in the motoneurons in the monkey brainstem and spinal cord. This antibody also revealed ChAT-positive terminal-like structures in the neuropils of lamina IX of the cervical spinal cord and interpeduncular nucleus.
