使用[3H]XCC（1,3-二丙基黄嘌呤的功能化类似物）对大鼠脑中腺苷A1受体进行放射自显影定位。
Autoradiographic localization of adenosine A1 receptors in rat brain using [3H]XCC, a functionalized congener of 1,3-dipropylxanthine.
作者信息
Jarvis M F, Jacobson K A, Williams M
机构信息
Research Department, CIBA-GEIGY Corporation, Summit, NJ 07901.
出版信息
Neurosci Lett. 1987 Oct 16;81(1-2):69-74. doi: 10.1016/0304-3940(87)90342-9.
Abstract
Quantitative autoradiography was used to visualize the anatomical distribution of adenosine receptors labeled by the carboxylic acid congener of 1,3-dipropylxanthine, 3H([3H]XCC ) in the rat brain. [3H]XCC was observed to specifically bind to rat brain sagittal sections in a heterogeneous pattern. Saturation experiments revealed that [3H]XCC binds with nanomolar affinity to 20-microns frozen tissue sections with the highest binding densities occurring in the hippocampus and cerebellum. Both the binding characteristics and regional receptor distribution obtained with [3H]XCC demonstrate the potential usefulness of this new ligand in the study of adenosine A1 receptors.
摘要
采用定量放射自显影技术观察1,3 - 二丙基黄嘌呤的羧酸类似物3Hphenyl - 1,3 - 二丙基黄嘌呤)([3H]XCC)标记的腺苷受体在大鼠脑内的解剖分布。观察到[3H]XCC以异质性模式特异性结合大鼠脑矢状切片。饱和实验表明，[3H]XCC以纳摩尔亲和力与20微米厚的冷冻组织切片结合，海马和小脑中的结合密度最高。用[3H]XCC获得的结合特性和区域受体分布均表明这种新配体在腺苷A1受体研究中的潜在用途。
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