Goodman R R, Synder S H
J Neurosci. 1982 Sep;2(9):1230-41. doi: 10.1523/JNEUROSCI.02-09-01230.1982.
Adenosine (A1) receptor binding sites have been localized in rat brain by an in vitro light microscopic autoradiographic method. The binding of [3H]N6-cyclohexyladenosine to slide-mounted rat brain tissue sections has the characteristics of A1 receptors. It is saturable with high affinity and has appropriate pharmacology and stereospecificity. The highest densities of adenosine receptors occur in the molecular layer of the cerebellum, the molecular and polymorphic layers of the hippocampus and dentate gyrus, the medial geniculate body, certain thalamic nuclei, and the lateral septum. High densities also are observed in certain layers of the cerebral cortex, the piriform cortex, the caudate-putamen, the nucleus accumbens, and the granule cell layer of the cerebellum. Most white matter areas, as well as certain gray matter areas, such as the hypothalamus, have negligible receptor concentrations. These localizations suggest possible central nervous system sites of action of adenosine.
通过体外光学显微镜放射自显影法已将腺苷（A1）受体结合位点定位在大鼠脑中。[3H]N6-环己基腺苷与载玻片上的大鼠脑组织切片的结合具有A1受体的特征。它具有高亲和力的饱和性，并具有适当的药理学和立体特异性。腺苷受体的最高密度出现在小脑分子层、海马和齿状回的分子层和多形层、内侧膝状体、某些丘脑核以及外侧隔区。在大脑皮层的某些层、梨状皮层、尾状核-壳核、伏隔核和小脑颗粒细胞层也观察到高密度。大多数白质区域以及某些灰质区域，如下丘脑，受体浓度可忽略不计。这些定位提示了腺苷在中枢神经系统中可能的作用部位。
