唾液酸参与大鼠脑突触体对多巴胺的高亲和力摄取。

Involvement of sialic acid in high-affinity uptake of dopamine by synaptosomes from rat brain.

作者信息

Zaleska M M, Erecińska M

机构信息

Department of Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, Philadelphia 19104.

出版信息

Neurosci Lett. 1987 Nov 10;82(1):107-12. doi: 10.1016/0304-3940(87)90179-0.

DOI:10.1016/0304-3940(87)90179-0

PMID:3696480

Abstract

The high-affinity, sodium-dependent uptake of dopamine (DA) was inhibited by the pretreatment of synaptosomes with neuraminidase from Vibrio cholerae. The inhibition was of a non-competitive type, resulting in a 40% decrease of Vmax. Neither basal nor depolarization-stimulated release of DA was affected. Treatment of synaptosomes with neuraminidase caused a 48% loss of sialic acid from the lipid-bound pool and a 80% decrease in the protein-bound fraction. The inhibition of DA uptake was found to be related linearly to the loss of sialic acid from the protein pool. It is postulated that a sialic acid moiety is involved in DA transport across the synaptosomal membrane.

摘要

用霍乱弧菌神经氨酸酶预处理突触体后，多巴胺（DA）的高亲和力、钠依赖性摄取受到抑制。这种抑制是非竞争性的，导致最大摄取速率（Vmax）降低40%。基础状态下和去极化刺激的DA释放均未受影响。用神经氨酸酶处理突触体导致脂质结合池中的唾液酸损失48%，蛋白质结合部分减少80%。发现DA摄取的抑制与蛋白质池中唾液酸的损失呈线性相关。据推测，唾液酸部分参与了DA跨突触体膜的转运。