Krause D, Debuch H
Institut für physiologische Chemie, Universität zu Köln, F.R.G.
Neurosci Lett. 1987 Nov 10;82(1):53-7. doi: 10.1016/0304-3940(87)90170-4.
Astrocytes of mouse cerebellum were grown in chemically defined medium for 14 days, subcultivated and grown in serum-containing medium for up to 14 days. After 14 days in defined medium the cells showed fatty degeneration from which they recovered histologically and chemically after one week in serum-supplemented medium. At this point 99% of the cells were glial fibrillary acidic protein-positive and synthesized phosphatidylinositol, phosphatidylserine and bis(monoacylglycero)phosphate besides other phospholipids.
