Teschner R, Löffler M, Schneider F
Z Naturforsch C Biosci. 1980 Jan-Feb;35(1-2):124-8. doi: 10.1515/znc-1980-1-223.
Total lipids of Ehrlich ascites tumor cells grown in nutrient medium supplemented with 1% bovine serum albumin instead of 15% native horse serum and rate of incorporation of [3H] acetate into total lipids are reduced, while the cholesterol content of the cells is higher than of controls grown in normal medium. The lower lipid content of cells cultured in albumin supplemented medium is explained by a lack of exogenous lipids, which are normally taken up from the serum containing nutrient medium. A several fold increase of incorporation of labelled choline and ethanolamine into the phospholipids of the cells grown in the modified medium is observed. We suggest, that the observed stimulation of the incorporation of phospholipid precursors reflects changes in the dynamic state of the membranes produced by serum depletion.
在添加1%牛血清白蛋白而非15%天然马血清的营养培养基中培养的艾氏腹水瘤细胞的总脂质以及[3H]乙酸盐掺入总脂质的速率降低，而细胞的胆固醇含量高于在正常培养基中生长的对照细胞。在添加白蛋白的培养基中培养的细胞脂质含量较低是由于缺乏外源性脂质，而外源性脂质通常是从含血清的营养培养基中摄取的。观察到在改良培养基中生长的细胞中，标记的胆碱和乙醇胺掺入磷脂的量增加了几倍。我们认为，观察到的磷脂前体掺入的刺激反映了血清缺乏导致的细胞膜动态状态的变化。
