Biochemistry Department, North Eastern Hill University, Shillong 793022, India.
Interdisciplinary School of Life Sciences, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Mar Pollut Bull. 2023 May;190:114842. doi: 10.1016/j.marpolbul.2023.114842. Epub 2023 Mar 23.
Selenium (Se) is an essential element for living systems, however, toxic at higher levels. In the present study, Dunaliella salina cells were exposed to different Se concentrations for their growth (EC 195 mg L-) as well as Se accumulation. The cells exposed to 50 mg L- Se showed photoautotrophic growth parallel to control and accumulated 65 μg Se g- DW. A decrease in photosynthetic quantum yield, chlorophyll content, and the increase in intracellular reactive oxygen species, proline content, and lipid peroxidation accompanied by higher neutral lipid accumulation, were recorded at higher Se level. The enzymes superoxide dismutase and catalase played a pivotal role in antioxidative defense. Heterogeneity in accumulated carotenoids at varying concentrations of selenium was prevalent. The cells exposed to 200 mg L- Se resulted in the disorganization of organelles. Thus, the Se enriched biomass obtained at 50 mg L- may be explored for bio-fortification of food and feed.
硒（Se）是生命系统所必需的元素，但在较高水平下则具有毒性。在本研究中，杜氏盐藻细胞暴露于不同浓度的硒中，以研究其生长（EC195mg/L-1）和硒积累情况。暴露于 50mg/L Se 的细胞表现出与对照平行的光自养生长，并积累了 65μg Se/g DW。在较高硒水平下，记录到光合量子产率、叶绿素含量下降，细胞内活性氧、脯氨酸含量增加，脂质过氧化作用增强，同时中性脂质积累增加。超氧化物歧化酶和过氧化氢酶等酶在抗氧化防御中起着关键作用。在不同浓度的硒中积累的类胡萝卜素存在异质性。暴露于 200mg/L Se 的细胞导致细胞器结构紊乱。因此，在 50mg/L Se 下获得的富含硒的生物质可能会被用于食品和饲料的生物强化。
