Liu Xiaojing, Chen Yaqian, Bu Tong, Deng Ziai, Zhao Lei, Tian Yanli, Jia Conghui, Li Yuechun, Wang Rong, Wang Jianlong, Zhang Daohong
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan, 450002, China.
Biosens Bioelectron. 2023 Jun 1;229:115239. doi: 10.1016/j.bios.2023.115239. Epub 2023 Mar 21.
Portable devices for on-site foodborne pathogens detection are urgently desirable. Lateral flow immunoassay (LFIA) provides an efficient strategy for pathogens detection, however, antibody labeling independence and detection reliability, are still challenging. Here, we report the development of a label-free LFIA with dual-readout using glucan-functionalized two-dimensional (2D) transition metal dichalcogenides (TMDs) tungsten disulfide (WS) as detection probes for sensitive detection of Salmonella enteritidis (S. enteritidis). In particular, glucan-functionalized WS, synthesized via liquid exfoliation, are reliable detection antibody candidates which served as antibody mimics for bacteria capturing. This LFIA has not only eliminated the intricate antibody labeling process and screening of paired antibodies in conventional LFIAs, but also promised dual-readout (colorimetric/Raman) for flexible detection. Under optimized conditions, this LFIA achieves selective detection of S. enteritidis with a low visual detection limit of 10 CFU/mL and a broad linear range of 10-10 CFU/mL. Additionally, the LFIA could be successfully applied in drinking water and milk with recoveries of 85%-109%. This work is desirable to expand the application of 2D TMDs in biosensors and offers a brand-new alternative protocol of detection antibodies in foodborne pathogens detection.
迫切需要用于现场检测食源性病原体的便携式设备。侧向流动免疫分析（LFIA）为病原体检测提供了一种有效的策略，然而，抗体标记的独立性和检测可靠性仍然具有挑战性。在此，我们报告了一种无标记的双读出LFIA的开发，该方法使用葡聚糖功能化的二维（2D）过渡金属二硫属化物（TMDs）二硫化钨（WS）作为检测探针，用于灵敏检测肠炎沙门氏菌（S. enteritidis）。特别地，通过液体剥离合成的葡聚糖功能化WS是可靠的检测抗体候选物，可作为捕获细菌的抗体模拟物。这种LFIA不仅消除了传统LFIA中复杂的抗体标记过程和配对抗体的筛选，而且还实现了双读出（比色/拉曼）以进行灵活检测。在优化条件下，这种LFIA能够选择性检测肠炎沙门氏菌，视觉检测下限低至10 CFU/mL，线性范围宽达10-10 CFU/mL。此外，该LFIA可成功应用于饮用水和牛奶检测，回收率为85%-109%。这项工作有望扩大二维TMDs在生物传感器中的应用，并为食源性病原体检测中的检测抗体提供一种全新的替代方案。
