Sha Yuanyuan, Zhou Linlin, Wang Zedi, Ding Ying, Lu Minrui, Xu Zhaoxian, Zhai Rui, Jin Mingjie
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Biorefinery Research Institution, Nanjing University of Science and Technology, Nanjing 210094, China.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Biorefinery Research Institution, Nanjing University of Science and Technology, Nanjing 210094, China.
J Biotechnol. 2023 Apr 10;367:42-52. doi: 10.1016/j.jbiotec.2023.03.006. Epub 2023 Mar 23.
Microbial tolerance to lignocellulose-derived inhibitors, such as aromatic acids, is critical for the economical production of biofuels and biochemicals. Here, adaptive laboratory evolution was applied to improve the tolerance of Yarrowia lipolytica to a representative aromatic acid inhibitor vanillic acid. The transcriptome profiling of evolved strain suggested that the tolerance could be related to the up-regulation of RNA processing and multidrug transporting pathways. Further analysis by reverse engineering confirmed that the amplification of YALI0_F13475g coding for transcriptional coactivator and YALI0_E25201g coding for multidrug transporter conferred tolerance not only to vanillic acid but also towards ferulic acid, p-coumaric acid, p-hydroxybenzoic acid and syringic acid. These findings suggested that regulation of RNA processing and multidrug transporting pathways may be important for enhanced aromatic acid tolerance in Y. lipolytica. This study provides valuable genetic information for robust strain construction for lignocellulosic biorefinery.
微生物对木质纤维素衍生抑制剂（如芳香酸）的耐受性对于生物燃料和生物化学品的经济生产至关重要。在此，应用适应性实验室进化来提高解脂耶氏酵母对代表性芳香酸抑制剂香草酸的耐受性。进化菌株的转录组分析表明，耐受性可能与RNA加工和多药转运途径的上调有关。通过逆向工程的进一步分析证实，编码转录共激活因子的YALI0_F13475g和编码多药转运蛋白的YALI0_E25201g的扩增不仅赋予了对香草酸的耐受性，还赋予了对阿魏酸、对香豆酸、对羟基苯甲酸和丁香酸的耐受性。这些发现表明，RNA加工和多药转运途径的调节可能对提高解脂耶氏酵母对芳香酸的耐受性很重要。本研究为木质纤维素生物炼制的稳健菌株构建提供了有价值的遗传信息。
