School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, China.
School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, China.
Bioresour Technol. 2021 Jun;329:124910. doi: 10.1016/j.biortech.2021.124910. Epub 2021 Feb 27.
Yarrowia lipolytica is an efficient oleaginous yeast, whereas its activity is typically reduced by inhibitors present in lignocellulosic hydrolysate. Understanding the response mechanism of Y. lipolytica to hydrolysate inhibitors and developing inhibitor tolerant strains are vital to lignocellulose valorization by this promising species. In this study, through adaptive laboratory evolution on three representative aromatic aldehyde inhibitors, evolved strains were obtained. Fermentation phenotype suggested that aromatic aldehydes conversion was one main reason for high tolerance of adapted strains. Transcriptome profiling analysis and reverse metabolic engineering confirmed that overexpressing the aldehyde ketone reductase gene YALI0_B07117g and aldehyde dehydrogenase gene YALI0_B01298g effectively converted aromatic aldehyde to corresponding alcohols and acids. The potential degradation pathways for aromatic aldehyde inhibitors in Y. lipolytica XYL+ were then discussed. This study provided insights to the aromatic aldehyde degradation in Y. lipolytica and a reliable basis for the development of aromatic aldehyde tolerant strains.
解脂耶氏酵母是一种高效的油脂酵母，但它的活性通常会被木质纤维素水解物中的抑制剂所抑制。了解解脂耶氏酵母对水解物抑制剂的响应机制，并开发出对抑制剂具有耐受性的菌株，对于该有前途的物种利用木质纤维素至关重要。在这项研究中，通过对三种代表性的芳香醛抑制剂进行适应性实验室进化，获得了进化菌株。发酵表型表明，芳香醛的转化是适应菌株高耐受性的一个主要原因。转录组谱分析和反向代谢工程证实，过表达醛酮还原酶基因 YALI0_B07117g 和醛脱氢酶基因 YALI0_B01298g 可以有效地将芳香醛转化为相应的醇和酸。然后讨论了解脂耶氏酵母 XYL+中芳香醛抑制剂的潜在降解途径。本研究为解脂耶氏酵母中芳香醛的降解提供了新的见解，并为芳香醛耐受菌株的开发提供了可靠的依据。
