空间代谢组学对口腔癌预后中免疫微环境的影响：一项临床报告。
Impact of spatial metabolomics on immune-microenvironment in oral cancer prognosis: a clinical report.
机构信息
Tata Medical Center, Newtown, Kolkata, 700 160, India.
CSIR-Institute of Genomics and Integrative Biology (IGIB), Mall Road, New Delhi, 110 007, India.
出版信息
Mol Cell Biochem. 2024 Jan;479(1):41-49. doi: 10.1007/s11010-023-04713-3. Epub 2023 Mar 26.
MALDI imaging for metabolites and immunohistochemistry for 38 immune markers was used to characterize the spatial biology of 2 primary oral tumours, one from a patient with an early recurrence (Tumour R), and the other from a patient with no recurrence 2 years after treatment completion (Tumour NR). Tumour R had an increased purine nucleotide metabolism in different regions of tumour and adenosine-mediated suppression of immune cells compared to Tumour NR. The differentially expressed markers in the different spatial locations in tumour R were CD33, CD163, TGF-β, COX2, PD-L1, CD8 and CD20. These results suggest that altered tumour metabolomics concomitant with a modified immune microenvironment could be a potential marker of recurrence.
MALDI 成像用于代谢物分析，免疫组织化学用于 38 种免疫标志物分析，用于描述 2 个原发口腔肿瘤的空间生物学特征，一个来自早期复发患者（肿瘤 R），另一个来自治疗完成 2 年后无复发患者（肿瘤 NR）。与肿瘤 NR 相比，肿瘤 R 的不同区域嘌呤核苷酸代谢增加，且腺苷介导的免疫细胞抑制增强。肿瘤 R 中不同空间位置的差异表达标志物为 CD33、CD163、TGF-β、COX2、PD-L1、CD8 和 CD20。这些结果表明，肿瘤代谢组学的改变伴随着免疫微环境的改变，可能是复发的潜在标志物。
Zotero 插件