Stasikowska-Kanicka Olga, Wągrowska-Danilewicz Małgorzata, Danilewicz Marian
Department of Nephropathology, Medical University of Lodz, Lodz, Poland.
Department of Pathomorphology, Medical University of Lodz, Lodz, Poland.
APMIS. 2018 Sep;126(9):732-738. doi: 10.1111/apm.12881.
Overexpression of inhibitory checkpoint PD1/PD-L1 plays an important role in carcinogenesis and patients prognosis. 70 cases of oral squamous cell carcinoma (OSCC), 23 cases of oral leukoplakia (OLK), and 19 control cases were immunohistochemically stained with anti-PD-L1, -CD8, and -CD163 antibodies. PD-L1 was expressed on dysplastic and subepithelial infiltrating cells of OLK as well as on cancer and tumor-infiltrating cells of OSCC. In OSCC, PD-L1 immunoexpression was significantly increased in comparison to OLK, and control groups. The correlative study showed significant correlations between the immunoexpression of PD-L1 and the number of CD8+, CD163+ cells in both OLK and OSCC groups. We found also significant negative correlation between the number of PD-L1+ infiltrating cells and the number of CD8+ cells in OSCC, and positive correlation between the number of PD-L1+ infiltrating cells and CD163+ cells in OLK and OSCC groups. In conclusion, our study indicate that CD163+ and CD8+ infiltrating cells influence the early and subsequent stages of oral carcinogenesis. We demonstrated also that studied tumors may evade the host immune system by PD-L1 immunoexpression not only on epithelial cells but on infiltrating cells as well.
抑制性检查点PD1/PD-L1的过表达在致癌作用和患者预后中起重要作用。用抗PD-L1、-CD8和-CD163抗体对70例口腔鳞状细胞癌（OSCC）、23例口腔白斑（OLK）和19例对照病例进行免疫组织化学染色。PD-L1在OLK的发育异常和上皮下浸润细胞以及OSCC的癌细胞和肿瘤浸润细胞上表达。在OSCC中，与OLK和对照组相比，PD-L1免疫表达显著增加。相关性研究表明，在OLK和OSCC组中，PD-L1的免疫表达与CD8+、CD163+细胞数量之间存在显著相关性。我们还发现，在OSCC中，PD-L1+浸润细胞数量与CD8+细胞数量之间存在显著负相关，在OLK和OSCC组中，PD-L1+浸润细胞数量与CD163+细胞数量之间存在正相关。总之，我们的研究表明，CD163+和CD8+浸润细胞影响口腔癌发生的早期和后续阶段。我们还证明，所研究的肿瘤不仅可以通过上皮细胞上的PD-L1免疫表达，还可以通过浸润细胞上的PD-L1免疫表达来逃避宿主免疫系统。
