口腔白斑病伴口腔黏膜下纤维性变的免疫微环境初步探讨：一项比较免疫组织化学研究。

The preliminary exploration of immune microenvironment in oral leukoplakia concomitant with oral submucosal fibrosis: A comparative immunohistochemical study.

机构信息

Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, People's Republic of China.

Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, People's Republic of China.

出版信息

J Oral Pathol Med. 2023 Aug;52(7):666-672. doi: 10.1111/jop.13434. Epub 2023 Apr 21.

DOI:10.1111/jop.13434

PMID:37084341

Abstract

BACKGROUND

Oral leukoplakia concomitant with oral submucous fibrosis is a high-risk oral potentially malignant disorder, but little is known about its immune microenvironment.

METHODS

Thirty samples of oral leukoplakia concomitant with oral submucous fibrosis, 30 oral leukoplakia samples, and 30 oral submucous fibrosis samples were collected from two hospitals. Immunohistochemistry was performed to analyze expression of T cell biomarkers [CD3, CD4, CD8, and Forkhead box P3 (Foxp3)], a B cell biomarker (CD20), macrophage biomarkers (CD68 and CD163), an immune inhibitory receptor ligand (PD-L1), and Ki-67.

RESULTS

The numbers of CD3 (p < 0.001), CD4 (p = 0.018), and CD8 (p = 0.031) cells in oral leukoplakia concomitant with oral submucous fibrosis were less than those in oral leukoplakia. The number of CD4 cells (p = 0.035) in oral leukoplakia concomitant with oral leukoplakia was higher than that in oral submucous fibrosis. More CD3 (p < 0.001), CD4 (p < 0.001), Foxp3 (p = 0.019), and CD163 (p = 0.029) cells were found in oral leukoplakia than in oral submucous fibrosis.

CONCLUSION

Various levels of immune infiltration were observed among oral leukoplakia concomitant with oral submucous fibrosis, oral leukoplakia, and oral submucous fibrosis. Characterization of the immune microenvironment may contribute to personalized immunotherapy.

摘要

背景

口腔白斑病伴口腔黏膜下纤维化为口腔高风险潜在恶性疾病，但对其免疫微环境知之甚少。

方法

从两家医院收集了 30 例口腔白斑病伴口腔黏膜下纤维化、30 例口腔白斑病和 30 例口腔黏膜下纤维化样本。采用免疫组织化学方法分析 T 细胞标志物（CD3、CD4、CD8 和叉头框 P3（Foxp3））、B 细胞标志物（CD20）、巨噬细胞标志物（CD68 和 CD163）、免疫抑制受体配体（PD-L1）和 Ki-67 的表达。

结果

口腔白斑病伴口腔黏膜下纤维化中 CD3（p<0.001）、CD4（p=0.018）和 CD8（p=0.031）细胞数量少于口腔白斑病。口腔白斑病伴口腔白斑病中 CD4 细胞数量（p=0.035）高于口腔黏膜下纤维化。口腔白斑病中 CD3（p<0.001）、CD4（p<0.001）、Foxp3（p=0.019）和 CD163（p=0.029）细胞数量多于口腔黏膜下纤维化。