Chi Jimei, Su Meng, Xue Bingjie, Cheng Lijun, Lian Zewei, Yun Yang, Yang Xu, Wang Xue, Xie Hongfei, Wang Huadong, Wang Yuan, Du Jie, Song Yanlin
Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences (ICCAS)/Beijing Engineering Research Center of Nanomaterials for Green Printing Technology, Beijing National Laboratory for Molecular Sciences (BNLMS), Beijing 100190, P. R. China.
University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
ACS Sens. 2023 Apr 28;8(4):1742-1749. doi: 10.1021/acssensors.3c00029. Epub 2023 Mar 26.
With the demand for point-of-care testing (POCT) in cardiovascular diseases, the detection of biomarkers in trace blood samples is of great significance in emergency medicine settings. Here, we demonstrated an all-printed photonic crystal microarray for POCT of protein markers (named "P4 microarray"). The paired nanobodies were printed as probes to target the soluble suppression of tumorigenicity 2 (sST2) as a certified cardiovascular protein marker. Benefiting from photonic crystal-enhanced fluorescence and integrated microarrays, quantitative detection of sST2 is 2 orders of magnitude lower than that of a traditional fluorescent immunoassay. The limit of detection is down to 10 pg/mL with the coefficient of variation being less than 8%. Detection of sST2 via fingertip blood is achieved in 10 min. Moreover, the P4 microarray after 180 days of storage at room temperature showed excellent stability for detection. This P4 microarray, as a convenient and reliable immunoassay for rapid and quantitative detection of protein markers in trace blood samples, has high sensitivity and strong storage stability, which hold great potential to advance cardiovascular precision medicine.
随着心血管疾病即时检测（POCT）需求的增加，在急诊医学环境中检测微量血样中的生物标志物具有重要意义。在此，我们展示了一种用于蛋白质标志物POCT的全印刷光子晶体微阵列（命名为“P4微阵列”）。将配对的纳米抗体作为探针进行印刷，以靶向可溶性肿瘤抑制因子2（sST2），sST2是一种经认证的心血管蛋白标志物。受益于光子晶体增强荧光和集成微阵列，sST2的定量检测比传统荧光免疫分析低2个数量级。检测限低至10 pg/mL，变异系数小于8%。通过指尖血检测sST2可在10分钟内完成。此外，P4微阵列在室温下储存180天后仍表现出优异的检测稳定性。这种P4微阵列作为一种方便可靠的免疫分析方法，可用于快速定量检测微量血样中的蛋白质标志物，具有高灵敏度和强储存稳定性，在推进心血管精准医学方面具有巨大潜力。
