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[皮层在海胆双胎发育中的形态发生作用]

[Morphogenetic role of the cortex in the development of twins in sea urchins].

作者信息

Drozdov A L, Sviatogor G P

出版信息

Ontogenez. 1987 Sep-Oct;18(5):540-6.

PMID:3696680
Abstract

The dynamics of development of Strongylocentrotus nudus sea urchin twins has been studied after centrifugation at 2,000 g for 5 min and incubation in hypotonic medium after fertilization till formation of the first cleavage furrow. Cortex rigidity was found to correlate with the frequency of twins occurrence. The largest amount of twins forms after centrifugation and hypotonic treatment for the first 1-6 min of zygote development. The twins may be obtained at 25-40 min of development by centrifugation in the presence of cytochalasin B (1 micrograms/ml). Morphogenetic role of cortex in development of sea urchin twins has been discussed.

摘要

在对光棘球海胆受精卵以2000g离心5分钟,并在受精后于低渗培养基中培养直至形成第一条卵裂沟后,研究了其双生幼体的发育动态。发现皮层硬度与双生幼体出现的频率相关。在合子发育的最初1 - 6分钟进行离心和低渗处理后,形成的双生幼体数量最多。在细胞松弛素B(1微克/毫升)存在的情况下,于发育25 - 40分钟时进行离心也可获得双生幼体。文中讨论了皮层在海胆双生幼体发育中的形态发生作用。

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