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模型细胞悬液的低场1H NMR参数化：扩散与弛豫研究

Low-field 1H NMR parameterization of model cell suspensions. A diffusion and relaxation study.

作者信息

Habina-Skrzyniarz Iwona, Krzyżak Artur T

机构信息

AGH University of Science and Technology, Krakow, Poland.

出版信息

Postepy Kardiol Interwencyjnej. 2022 Dec;18(4):392-398. doi: 10.5114/aic.2022.120994. Epub 2022 Nov 8.

DOI:10.5114/aic.2022.120994
PMID:36967842
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10031678/
Abstract

INTRODUCTION

Nuclear magnetic resonance (NMR) is a powerful method for the non-invasive study of a wide range of objects. Among its many characteristics, molecular diffusion can be examined without the need for any chemical or isotopic tracers by applying magnetic field gradients within the NMR sequence.

AIM

In our study, model cell suspensions were characterized by means of low-field (LF) (0.05 T) 1H NMR relaxometry. The proposed multi-parametric characterization based on independent 2D T1-T2 and D-T2 measurements was implemented to obtain a set of MR parameters as a specific signature for model cells.

MATERIAL AND METHODS

The D-T2 and T1-T2 correlation measurements were conducted on yeast samples with different amounts of added water. Signals from intracellular and extracellular water compartments and free water were identified on D-T2 maps and their diffusion coefficients were extracted.

RESULTS

Mean D_IC was equal to 8.4 × 10 m/s and mean D_EC ranged from 1.0 × 10 m/s to 1.65 × 10 m/s. T1/T2 ratio was calculated and for IC space values in the range of 4.2-5.3 were observed. Finally, we demonstrated the possibility of detecting signals from cells for the samples with a low concentration of cell suspensions or a small amount of the sample.

CONCLUSIONS

These findings are promising for more complex cell investigations and , without any contrast agents, applying solely biomarkers.

摘要

引言

核磁共振（NMR）是一种用于对多种物体进行非侵入性研究的强大方法。在其众多特性中，通过在NMR序列中应用磁场梯度，可以在无需任何化学或同位素示踪剂的情况下检测分子扩散。

目的

在我们的研究中，通过低场（LF）（0.05 T）1H NMR弛豫测量对模型细胞悬液进行了表征。基于独立的二维T1-T2和D-T2测量提出了多参数表征，以获得一组MR参数作为模型细胞的特定特征。

材料与方法

对添加不同水量的酵母样品进行D-T2和T1-T2相关性测量。在D-T2图上识别细胞内和细胞外水隔室以及自由水的信号，并提取其扩散系数。

结果

平均D_IC等于8.4×10 m/s，平均D_EC范围为1.0×10 m/s至1.65×10 m/s。计算了T1/T2比值，观察到IC空间值在4.2-5.3范围内。最后，我们证明了对于低浓度细胞悬液或少量样品的样本检测细胞信号的可能性。

结论

这些发现对于更复杂的细胞研究很有前景，并且在不使用任何造影剂的情况下，仅应用生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/cddb6b153531/PWKI-18-48141-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/87aa557ade2b/PWKI-18-48141-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/9b1e9ff802b3/PWKI-18-48141-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/8977aeccc970/PWKI-18-48141-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/cddb6b153531/PWKI-18-48141-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/87aa557ade2b/PWKI-18-48141-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/3a90169463ee/PWKI-18-48141-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/8e7d1e481363/PWKI-18-48141-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/9b1e9ff802b3/PWKI-18-48141-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9e3/10031678/cddb6b153531/PWKI-18-48141-g006.jpg

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Attempts at the Characterization of In-Cell Biophysical Processes Non-Invasively-Quantitative NMR Diffusometry of a Model Cellular System.
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INDIANA: An in-cell diffusion method to characterize the size, abundance and permeability of cells.印第安纳州：一种用于细胞内扩散方法，可用于描述细胞的大小、丰度和渗透性。
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