Identification of cell culture contamination by an unusual species of related to the cluster.

contamination is a significant problem in cell culture replication and maintenance. From more than 200 known species, a limited number of species have been detected in cell cultures, representing new species or variants that can escape detection systems. A qPCR commercial kit was used for detection in cell cultures. Furthermore, an amplified species was sequenced and summited for sequence assembly, clustering, and evolutionary analysis study. Our work has identified a new and unusual variant or species of that possesses a high degree of homology with species related with cluster. This variant is usually associated with cattle but has been detected contaminating a cell culture. testing (even for unusual species) in cell cultures is essential to ensure the validity and reproducibility of research that uses cell cultures and to ensure the quality of cell line deposits in biobanks. For this reason, it is necessary to perform continuous checks for the absence of in cell cultures and engage in the continuous adaptation of relevant detection systems.