Xia Ji, Chen Gao-Yang, Li You You, Chen Lu, Lu Dan
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, China.
Department of Oncology, The Second People's Hospital of Taizhou City, Taizhou, China.
Front Bioeng Biotechnol. 2023 Mar 2;11:1111866. doi: 10.3389/fbioe.2023.1111866. eCollection 2023.
Early, efficient and sensitive detection of serum markers in cervical cancer is very important for the treatment and prognosis to cervical cancer patients. In this paper, a SERS platform based on surface enhanced Raman scattering technology was proposed to quantitatively detect superoxide dismutase in serum of cervical cancer patients. Au-Ag nanoboxs array was made by oil-water interface self-assembly method as the trapping substrate. The single-layer Au-AgNBs array was verified by SERS for possessing excellent uniformity, selectivity and reproducibility. 4-aminothiophenol (4-ATP) was used as Raman signal molecule, it will be oxidized to dithiol azobenzene under the surface catalytic reaction with the condition of PH = 9 and laser irradiation. The quantitative detection of SOD could be achieved by calculating the change of characteristic peak ratio. When the concentration was from 10 U mL-160 U mL, the concentration of SOD could be accurately and quantitatively detected in human serum. The whole test was completed within 20 min and the limit of quantitation was 10 U mL. In addition, serum samples from the cervical cancer, the cervical intraepithelial neoplasia and healthy people were tested by the platform and the results were consistent with those of ELISA. The platform has great potential as a tool for early clinical screening of cervical cancer in the future.
早期、高效且灵敏地检测宫颈癌血清标志物对于宫颈癌患者的治疗和预后非常重要。本文提出了一种基于表面增强拉曼散射技术的表面增强拉曼散射（SERS）平台，用于定量检测宫颈癌患者血清中的超氧化物歧化酶。采用油水界面自组装法制备了金-银纳米盒阵列作为捕获基底。通过表面增强拉曼散射（SERS）验证了单层金-银纳米盒阵列具有优异的均匀性、选择性和重现性。使用4-氨基硫酚（4-ATP）作为拉曼信号分子，在pH = 9和激光照射条件下，其在表面催化反应中会被氧化为二硫醇偶氮苯。通过计算特征峰比值的变化可实现对超氧化物歧化酶（SOD）的定量检测。当浓度在10 U/mL至60 U/mL范围内时，能够在人血清中准确、定量地检测出超氧化物歧化酶（SOD）的浓度。整个检测在20分钟内完成，定量限为10 U/mL。此外，该平台对宫颈癌、宫颈上皮内瘤变和健康人的血清样本进行了检测，结果与酶联免疫吸附测定（ELISA）的结果一致。该平台在未来作为宫颈癌早期临床筛查工具具有巨大潜力。
