Antifungal Activity of an Endophytic Fungus DYSJ3 from Blume against .

An endophytic fungus strain DYSJ3 was isolated from a stem of Blume, which was identified as based on the morphological characteristics, internal transcribed spacer (ITS) and calmodulin gene sequences analyses. DYSJ3 exhibited strong antagonistic activity against , and f. sp. with the inhibition rates of 61.9, 51.2 and 55.3% respectively. The antifungal metabolites mainly existed in the mycelium of DYSJ3, and its mycelial crude extract (CE) had broad-spectrum antifungal activities against plant pathogenic fungi. The CE had a good thermal stability, and the inhibition rate of 100 µg/mL CE against was above 70.0% after disposing at 120 °C for 1 h. Five secondary metabolites were isolated from the CE and identified as averufanin, ergosterol peroxide, versicolorin B, averythrin and sterigmatocystin. Activity evaluation showed versicolorin B exhibited inhibitory effects on the mycelial growth and conidial germination of , and sterigmatocystin had a weak inhibitory effect on the mycelial growth of .