Departments of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
J Zoo Wildl Med. 2023 Mar;54(1):202-210. doi: 10.1638/2022-0071.
This case series describes the use of confocal microscopy in the diagnosis and treatment of mycotic keratitis in two owls (one , one ) and one woodcock (). Each bird was at increased risk of fungal infection due to recent injury or stress. Ophthalmic findings in all birds included blepharospasm, ocular discharge, ulcerative keratitis, white or yellow corneal plaques, and anterior uveitis. Fungal hyphae were identified in corneal samples from all three eyes examined cytologically and in all three eyes by using confocal microscopy. was isolated from a corneal culture in one bird. Despite medical treatment, progressive ocular disease prompted enucleation in two birds. Fungal hyphae were detected by histopathology in one of the two enucleated eyes. confocal microscopy aided the diagnosis of fungal keratitis in all birds and was the only diagnostic method that allowed immediate, real-time quantification of the extent (area and depth) and severity of mycotic keratitis.
本病例系列描述了共聚焦显微镜在两只猫头鹰（一只是 ，另一只是 ）和一只丘鹬（）的真菌性角膜炎的诊断和治疗中的应用。由于最近受伤或压力，每只鸟都有更高的真菌感染风险。所有鸟类的眼部发现包括眼睑痉挛、眼部分泌物、溃疡性角膜炎、白色或黄色角膜斑块和前葡萄膜炎。通过细胞学检查和共聚焦显微镜检查，从三只受检眼中的两只眼的角膜样本中均发现了真菌菌丝。从一只鸟的角膜培养物中分离出了 。尽管进行了药物治疗，但两只鸟的眼部疾病仍在进展，导致眼球摘除。在两只眼球摘除的眼睛之一中，通过组织病理学检测到了真菌菌丝。共聚焦显微镜有助于所有鸟类的真菌性角膜炎的诊断，并且是唯一可以立即实时定量评估真菌性角膜炎的范围（面积和深度）和严重程度的诊断方法。
