Department of Chemistry, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
Basic Research Laboratory, Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
ACS Appl Bio Mater. 2023 Apr 17;6(4):1621-1628. doi: 10.1021/acsabm.3c00045. Epub 2023 Mar 27.
The lethality of the bovine viral diarrhea virus (BVDV) in cattle involves inapparent infection and various, typically subclinical, syndromes. Cattle of all ages are vulnerable to infection with the virus. It also causes considerable economic losses, primarily due to reduced reproductive performance. In the absence of treatment that can completely cure infected animals, detection of BVDV relies on highly sensitive and selective diagnosis methods. In this study, an electrochemical detection system was developed as a useful and sensitive system for the detection of BVDV to suggest the direction of diagnostic technology through the development of conductive nanoparticle synthesis. As a countermeasure, a more sensitive and rapid BVDV detection system was developed using the synthesis of electroconductive nanomaterials black phosphorus (BP) and gold nanoparticle (AuNP). To increase the conductivity effect, AuNP was synthesized on the BP surface, and the stability of BP was improved by using dopamine self-polymerization. Moreover, its characterizations, electrical conductivity, selectivity, and sensitivity toward BVDV also have been investigated. The BP@AuNP-peptide-based BVDV electrochemical sensor exhibited a low detection limit of 0.59 copies mL with high selectivity and long-term stability (retaining 95% of its initial performance over 30 days).
牛病毒性腹泻病毒（BVDV）在牛中的致死性涉及无症状感染和各种通常为亚临床的综合征。所有年龄段的牛都容易感染该病毒。它还会导致巨大的经济损失，主要是由于繁殖性能下降。由于没有可以完全治愈感染动物的治疗方法，因此 BVDV 的检测依赖于高度敏感和选择性的诊断方法。在这项研究中，开发了一种电化学检测系统，作为一种有用且敏感的 BVDV 检测系统，通过开发导电纳米粒子合成来为诊断技术的发展提供方向。作为对策，使用导电纳米材料黑磷（BP）和金纳米粒子（AuNP）的合成开发了更敏感和快速的 BVDV 检测系统。为了提高导电性效果，在 BP 表面合成了 AuNP，并通过多巴胺自聚合提高了 BP 的稳定性。此外，还对其进行了特性、电导率、选择性和对 BVDV 的敏感性研究。基于 BP@AuNP-肽的 BVDV 电化学传感器的检测限低至 0.59 拷贝 mL，具有高选择性和长期稳定性（在 30 天内保留了初始性能的 95%）。
