Zhao Yuhan, E Zhiqiang, Jiao Anhui, Sun Zhaoyang, Zhang Hongbo, Wang Haijun, Fang Nanzhu, Gao Qingshan, Jin Qingguo
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China; College of Agriculture, Yanbian University, Yanji, 133002, China; Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, Yanji, 133002, China.
Zhejiang Zhongxing Animal Husbandry Technology Co. Ltd., Wenzhou, 325000, China.
Theriogenology. 2023 Jun;203:53-60. doi: 10.1016/j.theriogenology.2023.03.015. Epub 2023 Mar 21.
Strategies for improving the quality of oocytes have important theoretical and practical significance for increasing the efficiency of livestock breeding. In this respect, the accumulation of reactive oxygen species (ROS) is a major factor affecting the development of oocytes and embryos. This study investigated the effects of Dendrobium nobile extract (DNE) on the in vitro maturation of bovine oocytes and embryonic development after IVF. DNE is an extract from Dendrobium rhizomes that contains alkaloids with anti-inflammatory, anti-cancer and anti-ageing functions. Various concentrations of DNE (0, 5, 10, 20 and 50 μmol/L) were added during oocyte maturation in vitro, and we found that 10 μmol/L of DNE remarkably increased the oocyte maturation rate, the subsequent blastocyst formation rate and embryo quality. Further, we found that DNE treatment decreased the frequency of spindle/chromosome defects and ROS and increased the oocyte glutathione and mitochondrial membrane potential in oocytes. Moreover, DNE upregulated the expression of oxidative stress-related genes (Sirt1, Sirt2, Sirt3 and Sod1) in oocytes and apoptosis-related genes (Caspase-3, Caspase-4, Bax, Bcl-xl and Survivin) in blastocysts. These results suggest that DNE supplementation can promote oocyte maturation and subsequent embryonic development by regulating redox reactions and inhibiting embryonic apoptosis.
提高卵母细胞质量的策略对于提高家畜繁殖效率具有重要的理论和实践意义。在这方面，活性氧（ROS）的积累是影响卵母细胞和胚胎发育的主要因素。本研究探讨了铁皮石斛提取物（DNE）对牛卵母细胞体外成熟及体外受精后胚胎发育的影响。DNE是从石斛根茎中提取的一种提取物，含有具有抗炎、抗癌和抗衰老功能的生物碱。在卵母细胞体外成熟过程中添加不同浓度的DNE（0、5、10、20和50 μmol/L），我们发现10 μmol/L的DNE显著提高了卵母细胞成熟率、随后的囊胚形成率和胚胎质量。此外，我们发现DNE处理降低了纺锤体/染色体缺陷和ROS的发生率，并增加了卵母细胞中的谷胱甘肽和线粒体膜电位。此外，DNE上调了卵母细胞中氧化应激相关基因（Sirt1、Sirt2、Sirt3和Sod1）以及囊胚中凋亡相关基因（Caspase-3、Caspase-4、Bax、Bcl-xl和Survivin）的表达。这些结果表明，补充DNE可以通过调节氧化还原反应和抑制胚胎凋亡来促进卵母细胞成熟和随后的胚胎发育。
