TAT 基因多态性及其与番鸭（Cairina Moschata）生产性状的关系。

TAT gene polymorphism and its relationship with production traits in Muscovy ducks (Cairina Moschata).

机构信息

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.

College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, 666 Wusu Road, Lin'an 311300, China.

出版信息

Poult Sci. 2023 May;102(5):102551. doi: 10.1016/j.psj.2023.102551. Epub 2023 Jan 30.

DOI:10.1016/j.psj.2023.102551

PMID:36972669

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10050636/

Abstract

In a previous study, the laying pattern of Muscovy duck was explored by macro-fitting the laying curve of Muscovy duck, and transcriptome sequencing technique of the ovarian tissues was used to screen the egg-related gene "TAT." Moreover, recent results have shown that TAT is expressed in organs such as oviduct, ovary, and testis. The objective of this study is to examine the effect of TAT gene on egg production traits of Muscovy ducks. First, the expression levels of TAT gene in highest producing (HP) and lowest producing (LP) in 3 tissues related to reproduction were examined, and the results indicated that the expression of TAT gene in hypothalamus was significantly different between HP and LP groups. Then, 6 SNP loci (g. 120G>T, g, 122G>A, g, 254G> A, g. 270C >T, g, 312G>A, and g. 341C>A) were detected in TAT gene. Further, association analysis between the six SNP loci of TAT gene and egg production traits of 652 individual Muscovy ducks was done. The results showed that g. 254G>A and g. 270C>T were significantly correlated (P < 0.05 or 0.001) with the egg production traits of Muscovy ducks. This study elucidated the molecular mechanism that TAT gene might be regulating the egg production traits of Muscovy ducks.

摘要

在之前的研究中，通过对麝香鸭产蛋曲线进行宏观拟合，探索了麝香鸭的产蛋模式，并利用卵巢组织的转录组测序技术筛选出与蛋相关的基因“TAT”。此外，最近的研究结果表明，TAT 在输卵管、卵巢和睾丸等器官中表达。本研究旨在研究 TAT 基因对麝香鸭产蛋性能的影响。首先，检测了与生殖相关的 3 种组织中 TAT 基因在高生产（HP）和低生产（LP）个体中的表达水平，结果表明 TAT 基因在下丘脑中的表达在 HP 和 LP 组之间存在显著差异。然后，在 TAT 基因中检测到 6 个 SNP 位点（g.120G>T、g.122G>A、g.254G>A、g.270C>T、g.312G>A 和 g.341C>A）。进一步对 TAT 基因的 6 个 SNP 位点与 652 只个体麝香鸭产蛋性能进行关联分析。结果表明，g.254G>A 和 g.270C>T 与麝香鸭的产蛋性能显著相关（P < 0.05 或 0.001）。本研究阐明了 TAT 基因可能调节麝香鸭产蛋性能的分子机制。