Li Qingqing, Xu Mengwei, Wu Fengju, Guo Ziyi, Yang Ning, Li Lin, Wen Weie, Xu Delin
Department of Medical Instrumental Analysis, Zunyi Medical University, Zunyi, 563099, Guizhou, China.
Department of Cell Biology, Zunyi Medical University, Zunyi, 563099, Guizhou, China.
Adv Biotechnol (Singap). 2024 Jul 16;2(3):25. doi: 10.1007/s44307-024-00032-w.
Militarine is a monomer molecule with abundant and distinctive biological properties, also the lead member of secondary metabolites in Bletilla striata, while its biosynthesis mechanism is still unknown. To improve the production efficiency of militarine, sodium acetate and salicylic acid (SA) were introduced as elicitors into the suspension-cultured callus of B. striata. Subsequently, samples were taken from callus at different culturing stages to investigate the synthesis mechanisms of militarine in B. striata through integrated metabolomics and transcriptomics. Metabolomics analysis revealed that acetate ions promoted militarine synthesis, while SA had an inhibitory effect. Additionally, regulators such as ferulic acid, 2-hydroxy-3-phenylpropionic acid, and cis-beta-D-Glucosyl-2-hydroxycinnamate were identified as influencing militarine synthesis. Transcriptomics analysis indicated that the expression levels of genes involved in phenylalanine metabolism, phenylpropanoid biosynthesis, and tyrosine metabolism were correlated with militarine content. This study sheds light on the regulatory mechanism of militarine biosynthesis in plants. The results suggested that acetate ions and SA impact militarine synthesis through specific metabolic pathways and gene expression changes. This knowledge serves as a foundation for future research on militarine biosynthesis and its industrial production.
紫珠素是一种具有丰富独特生物学特性的单体分子，也是白及次生代谢产物中的主要成分，但其生物合成机制尚不清楚。为了提高紫珠素的生产效率，将醋酸钠和水杨酸（SA）作为诱导子引入白及悬浮培养愈伤组织中。随后，在不同培养阶段从愈伤组织中取样，通过整合代谢组学和转录组学研究白及中紫珠素的合成机制。代谢组学分析表明，醋酸根离子促进紫珠素合成，而SA具有抑制作用。此外，阿魏酸、2-羟基-3-苯基丙酸和顺式-β-D-葡萄糖基-2-羟基肉桂酸等调节因子被确定为影响紫珠素合成的因素。转录组学分析表明，参与苯丙氨酸代谢、苯丙烷生物合成和酪氨酸代谢的基因表达水平与紫珠素含量相关。本研究揭示了植物中紫珠素生物合成的调控机制。结果表明，醋酸根离子和SA通过特定的代谢途径和基因表达变化影响紫珠素合成。这些知识为未来紫珠素生物合成及其工业化生产的研究奠定了基础。
